Case-control study of individuals with discrepant nucleocapsid and spike protein SARS-CoV-2 IgG results (Publications)
BACKGROUND: Laboratory-based methods for SARS-CoV-2 antibody detection vary widely in performance. However, there are limited prospectively-collected data on assay performance, and minimal clinical in
Shared B cell memory to coronaviruses and other pathogens varies in human age groups and tissues (Publications)
Vaccination and infection promote the formation, tissue distribution, and clonal evolution of B cells, which encode humoral immune memory. We evaluated pediatric and adult blood and deceased adult org
Plasma as an alternative COVID-19 diagnostic specimen in a hospitalized patient negative for SARS-CoV-2 by nasopharyngeal swab (Publications)
We present the case of an inpatient with pneumonia and repeatedly negative nasopharyngeal SARS-CoV-2 testing. In such challenging cases, alternative diagnostic options include lower respiratory tract
Adjuvanting a subunit COVID-19 vaccine to induce protective immunity (Publications)
The development of a portfolio of COVID-19 vaccines to vaccinate the global population remains an urgent public health imperative(1). Here we demonstrate the capacity of a subunit vaccine, comprising
Increased viral variants in children and young adults with impaired humoral immunity and persistent SARS-CoV-2 infection: a consecutive case series (Publications)
BACKGROUND: There is increasing concern that persistent infection of SARS-CoV-2 within immunocompromised hosts could serve as a reservoir for mutation accumulation and subsequent emergence of novel st
Evaluation of SARS-CoV-2 total antibody detection via a lateral flow nanoparticle fluorescence immunoassay (Publications)
BACKGROUND: The coronavirus disease 2019 (COVID-19) endgame may benefit from simple, accurate antibody testing to characterize seroprevalence and immunization coverage. OBJECTIVES: To evaluate the per
Efficient identification of high-titer anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody plasma samples by pooling method (Publications)
CONTEXT.-: The ongoing COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has elicited a surge in demand for serologic testing to identify previously infected
Use of outpatient-derived COVID-19 convalescent plasma in COVID-19 patients before seroconversion (Publications)
BACKGROUND: Transfusion of COVID-19 convalescent plasma (CCP) containing high titers of anti-SARS-CoV-2 antibodies serves as therapy for COVID-19 patients. Transfusions early during disease course was
SARS-CoV-2 nucleocapsid plasma antigen for diagnosis and monitoring of COVID-19 (Publications)
BACKGROUND: Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen in blood has been described, but the diagnostic and prognostic role of antigenemia is not wel
Direct comparison of antibody responses to four SARS-CoV-2 vaccines in Mongolia (Publications)
Different SARS-CoV-2 vaccines are approved in various countries, but few direct comparisons of the antibody responses they stimulate have been reported. We collected plasma specimens in July 2021 from
