Increased detection of invasive enteropathogenic bacteria in pre-incubated blood culture materials by real-time PCR in comparison with automated... (Publications)
BACKGROUND: Invasive enteropathogenic bacteria can cause systemic infections. Data from studies with PCR detection suggest, at least for Salmonella enterica, that blood culture may lead to underestima
16S rRNA gene sequence-based identification of bacteria in automatically incubated blood culture materials from tropical sub-Saharan Africa (Publications)
BACKGROUND: The quality of microbiological diagnostic procedures depends on pre-analytic conditions. We compared the results of 16S rRNA gene PCR and sequencing from automatically incubated blood cult
Rapid discrimination of <em>Haemophilus influenzae</em>, <em>H. parainfluenzae</em>, and<em> H. haemolyticus</em> by fluorescence<em> in situ</em>... (Publications)
BACKGROUND: Due to considerable differences in pathogenicity, Haemophilus influenzae, H. parainfluenzae and H. haemolyticus have to be reliably discriminated in routine diagnostics. Retrospective anal
Rapid identification of <em>Acinetobacter</em> spp. by fluorescence <em>in situ</em> hybridization (FISH) from colony and blood culture material (Publications)
Multi-drug-resistant strains of the Acinetobacter baumannii complex cause nosocomial infections. Rapid identification of Acinetobacter spp. is desirable in order to facilitate therapeutic or hygiene d
Identification of lymphogranuloma venereum-associated <em>Chlamydia trachomatis</em> serovars by fluorescence <em>in situ</em> hybridisation--a... (Publications)
We describe a proof-of-principle evaluation of a fluorescence in situ hybridisation (FISH) procedure to identify Chlamydia trachomatis serovars L1-L3, the causative agents of lymphogranuloma venereum,
Rapid identification of yeast by fluorescence<em> in situ</em> hybridisation from broth and blood cultures (Publications)
Candida species including species other than Candida albicans are of importance as causative agents of sepsis in intensive-care units, requiring prompt initiation of targeted therapy. While fluconazol
Fluorescence in situ hybridization (FISH) for rapid identification of <em>Salmonella</em> spp. from agar and blood culture broth--an option for the... (Publications)
BACKGROUND: Salmonella enterica is an important cause of diarrhea with the potential to cause systemic infection including sepsis, particularly in the tropics. Sepsis in particular requires quick and
Influence of parasite density and sample storage time on the reliability of <em>Entamoeba histolytica</em>-specific PCR from formalin-fixed and... (Publications)
We report on the reliability of polymerase chain reaction (PCR) for the detection of Entamoeba histolytica from formalin-fixed, paraffin-embedded tissue in comparison with microscopy and have determin
Serological survey of HIV and syphilis in pregnant women in Madagascar (Publications)
OBJECTIVES: Peripartal transmission of human immunodeficiency virus (HIV) and Treponema pallidum, the causative agent of syphilis, leads to severe consequences for newborns. Preventive measures requir
Detection of resistance to macrolides in thermotolerant <em>Campylobacter</em> species by fluorescence in situ hybridization (Publications)
The resistance of enteritis-causing Campylobacter strains to erythromycin is an emerging problem. We therefore evaluated fluorescence in situ hybridization (FISH) for the rapid detection of resistance