Using a mobile nanopore sequencing lab for end-to-end genomic surveillance of <em>Plasmodium falciparum</em>: a feasibility study (Publications)
Genomic epidemiology holds promise for malaria control and elimination efforts, for example by informing on Plasmodium falciparum genetic diversity and prevalence of mutations conferring anti-malarial [...] drug resistance. Limited sequencing infrastructure in many malaria-endemic areas prevents the rapid generation of genomic data. To address these issues, we developed and validated assays for P. falciparum [...] resistance markers. We developed a bioinformatics workflow that allows genotyping...
Melissa Penny speaks about the beauty of disease modelling (Blog)
https://www.swisstph.ch/en/impact-stories/the-beauty-of-disease-modelling/blog-id/25
disease modelling for over 15 years. She joins us today to discuss the first malaria vaccine, disease modelling and the future of malaria eradication.
Rapid diagnostic tests for non-malarial febrile illness in the tropics (Publications)
of rapid diagnostic tests (RDTs) for malaria has highlighted the decreasing proportion of malaria-attributable illness in endemic areas. Unfortunately, once malaria is excluded, there are few accessible [...] pressing need for sensitive pathogen-detection-based RDTs, as have been successfully developed for malaria and dengue. Ultimately, integration of RDTs into a validated syndromic approach to tropical fevers
Rapid diagnostic tests for non-malarial febrile illness in the tropics (Publications)
of rapid diagnostic tests (RDTs) for malaria has highlighted the decreasing proportion of malaria-attributable illness in endemic areas. Unfortunately, once malaria is excluded, there are few accessible [...] pressing need for sensitive pathogen-detection-based RDTs, as have been successfully developed for malaria and dengue. Ultimately, integration of RDTs into a validated syndromic approach to tropical fevers
Immunoglobulin G subclass-specific responses against <em>Plasmodium falciparum</em> merozoite antigens are associated with control of parasitemia and... (Publications)
merozoite antigens play a role in protection from malaria, although the precise targets and mechanisms mediating immunity remain unclear. Different malaria antigens induce distinct immunoglobulin G (IgG) [...] (IgG) subclass responses, but the importance of different responses in protective immunity from malaria is not known and the factors determining subclass responses in vivo are poorly understood. We examined [...] AMA-1-specific IgG3 and MSP1-19-specific IgG1 were strongly predictive of a...
Immunoglobulin G subclass-specific responses against <em>Plasmodium falciparum</em> merozoite antigens are associated with control of parasitemia and... (Publications)
merozoite antigens play a role in protection from malaria, although the precise targets and mechanisms mediating immunity remain unclear. Different malaria antigens induce distinct immunoglobulin G (IgG) [...] (IgG) subclass responses, but the importance of different responses in protective immunity from malaria is not known and the factors determining subclass responses in vivo are poorly understood. We examined [...] AMA-1-specific IgG3 and MSP1-19-specific IgG1 were strongly predictive of a...
A multiplex qPCR approach for detection of <em>pfhrp2 </em>and <em>pfhrp3</em> gene deletions in multiple strain infections of <em>Plasmodium... (Publications)
The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic [...] blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput
Differential expression of var gene groups is associated with morbidity caused by <em>Plasmodium falciparum </em>infection in Tanzanian children (Publications)
uncomplicated, and asymptomatic malaria. Transcripts of var group A and B genes were more abundant in patients with severe malaria than in patients with uncomplicated malaria. In general, the transcript [...] abundances of var group A and B genes were higher for children with clinical malaria than for children with asymptomatic infections. The var group C and var1-like transcript abundances were similar between
Differential expression of var gene groups is associated with morbidity caused by <em>Plasmodium falciparum </em>infection in Tanzanian children (Publications)
uncomplicated, and asymptomatic malaria. Transcripts of var group A and B genes were more abundant in patients with severe malaria than in patients with uncomplicated malaria. In general, the transcript [...] abundances of var group A and B genes were higher for children with clinical malaria than for children with asymptomatic infections. The var group C and var1-like transcript abundances were similar between
A multiplex qPCR approach for detection of <em>pfhrp2 </em>and <em>pfhrp3</em> gene deletions in multiple strain infections of <em>Plasmodium... (Publications)
The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic [...] blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput