Recombinant expression of trypanosome surface glycoproteins in <em>Pichia pastoris</em> for the diagnosis of <em>Trypanosoma evansi</em> infection (Publications)
Pichia (P.) pastoris. The M5 strain of this yeast has an engineered N-glycosylation pathway resulting in homogenous Man5GlcNAc2 N-glycosylation which resembles the predominant Man9-5GlcNAc2 oligomannose structures
Antiprotozoal isoflavan quinones from <em>Abrus precatorius</em> ssp. <em>africanus</em> (Publications)
and three isoflavan quinones, abruquinone I (3), abruquinone B (4), and 7,8,3''5'-tetramethoxyisoflavan-1',4'-quinone (5). Compounds 1 and 3 were new natural products. The absolute configuration of compounds [...] values of 0.30 and 0.16 microM, respectively) and good selectivity (selectivity indices of 73.7 and 50.5, respectively)
Dioncophyllines C2, D2, and F and related naphthylisoquinoline alkaloids from the Congolese liana <em>Ancistrocladus ileboensis</em> with potent... (Publications)
Dioncophylline F (1), the first 5,8'-coupled dioncophyllaceous alkaloid (i.e., lacking an oxygen function at C-6 and possessing an R-configuration at C-3), was isolated from the recently described Congolese [...] semistable and, thus, slowly interconverting atropo-diastereomers, whereas dioncophylline C2 (2), with its 5,1'-linkage, is configurationally stable at the axis. Eight further known naphthylisoquinolines were [...] isolated from A. ileboensis, among them dioncophylline A (P-10), its...
Seroprevalence survey of brucellosis among rural people in Mongolia (Publications)
were being older than 45 years (adjusted odds ratio [AOR] = 6.9, 95% CI = 5.1-8.7) and being a veterinarian (AOR = 2.8, 95% CI = 1.5-5.0). CONCLUSION: Our study confirms that human brucellosis seroprevalence
Seroprevalence of Rift Valley fever, Q fever, and brucellosis in ruminants on the southeastern shore of Lake Chad (Publications)
and 10.8% (95% CI 3.0-18.5) in sheep. For brucellosis and Q fever, only cattle samples from islands were analyzed. For Q fever, the apparent seroprevalence was 7.8% (95% CI 5.6-10.1). For brucellosis, [...] the RBT showed a prevalence of 5.7% (95% CI 3.8-7.6), and ELISA showed 11.9% (95% CI 9.3-14.6) with a kappa value of 0.53 showing a moderate agreement between the two tests. This study confirms the presence
The epidemiology of multiple <em>Plasmodium falciparum</em> infections. 10. Effect of insecticide-treated bed nets on the dynamics of multiple... (Publications)
Plasmodium falciparum merozoite surface protein 2 (msp2) gene were studied in 120 children aged 5 months to 2.5 years, in a randomized controlled trial of insecticide-treated bed nets (ITNs) in Kiberege village [...] polymerase chain reaction-restriction fragment length polymorphism analysis in any one sample of 0.5 microL of packed peripheral blood cells. In children less than 18 months old this sensitivity was 61%
Pacing pattern and speed skating performance in competitive long-distance events (Publications)
in lap times were significant in each instance for women's 3,000 m (p < 0.001). For the 5,000 m races, laps 5-12 in women and laps 8-12 in men were slower compared with previous laps (p < 0.001, for both [...] complete World Cup series. Elite skaters competed in long-distance races of different length (3,000, 5,000, and 10,000 m) and location (low/high altitude) where distribution of lap times were analyzed.
Incidence and predictors of immune reconstitution inflammatory syndrome in a rural area of Mozambique (Publications)
patients developed IRIS [26.5%, incidence rate 3.1 cases/100 persons-month of ART (95% CI 2.2-4.3)]. Median time to IRIS onset was 62 days from ART initiation (IQR 35.5-93.5). Twenty-five cases (69.4%)
Estimating population and livestock density of mobile pastoralists and sedentary settlements in the south-eastern Lake Chad area (Publications)
were estimated to live in sedentary villages. In the mobile communities, we found 5.9 people per km2 at the beginning and 17.5 people per km2 at the end of the dry season. We recorded per km2 on average 21 [...] 21.0 cattle and 31.6 small ruminants in the sedentary villages and 66.1 cattle and 102.5 small ruminants in the mobile communities, which amounts to a mean of 86.6 tropical livestock units during the dry
Safety and immunogenicity of radiation-attenuated PfSPZ vaccine in equatoguinean infants, children, and adults (Publications)
ed Plasmodium falciparum sporozoites (PfSPZ) Vaccine has demonstrated safety and immunogenicity in 5-month-old to 50-year-old Africans in multiple trials. Except for one, each trial has restricted enrollment [...] intervals in a randomized, double-blind, placebo-controlled trial stratified by age (6-11 months and 1-5, 6-10, 11-17, 18-35, and 36-61 years). All doses were successfully administered. In all, 192/207 injections [...] occurred in 67/69 vaccinees (97%) and 0/15 controls. Median...