The Parasite Reduction Ratio (PRR) assay version 2: standardized assessment of <em>Plasmodium falciparum </em>viability after antimalarial... (Publications)
fast rate of parasite killing or clearance. To determine these parameters, it is essential to discriminate viable from nonviable parasites, which is complicated by the fact that viable parasites can be me [...] reliably discriminate between viable and nonviable parasites. Conversely, the in vitro parasite reduction ratio (PRR) assay is able to measure viable parasites with high sensitivity. It provides valuable p [...] With artemisinin-resistant Plasmodium falciparum parasites emerging in...
The ApiAP2 factor PfAP2-HC is an integral component of heterochromatin in the malaria parasite <em>Plasmodium falciparum</em> (Publications)
Malaria parasites undergo a complex life cycle in the human host and the mosquito vector. The ApiAP2 family of DNA-binding proteins plays a dominant role in parasite development and life cycle progression [...] maintenance. In summary, our findings reveal PfAP2-HC as a core component of heterochromatin in malaria parasites and identify unexpected properties and substantial functional divergence among the members of the
A protocol for HPLC-based activity profiling for natural products with activities against tropical parasites (Publications)
micro-fractions, and a suitable dilution scheme permitted parallel activity profiling against three parasites from a single HPLC injection. The protocol was validated with extracts and positive controls such
Characterization of a defensin from the sand fly <em>Phlebotomus duboscqi</em> induced by challenge with bacteria or the protozoan parasite... (Publications)
Phlebotomus duboscqi, after challenge by injected bacteria or feeding with bacteria or the protozoan parasite Leishmania major. A new hemolymph peptide with antimicrobial activity was identified and shown to [...] raises the possibility that this antimicrobial peptide might play a key role in the development of parasitic infections
