Immuno-gold silver staining assays on capillary-driven microfluidics for the detection of malaria antigens (Publications)
a lack of highly sensitive malaria RDTs that can detect low antigen concentration at the onset of infection. Here, we present a strategy to improve the sensitivity of malaria RDTs by using capillary-driven [...] al to the concentration of antigen in a sample. We illustrate this method using the recombinant malaria antigen Plasmodium falciparum histidine-rich-protein 2 (rPfHRP2) spiked in human serum. This antigen [...] min, which is approaching the desired sensitivity needed in the Target...
Hemozoin-catalyzed precipitation polymerization as an assay for malaria diagnosis (Publications)
Methods to diagnose malaria are of paramount interest to eradicate the disease. Current methods have severe limitations, as they are either costly or not sensitive enough to detect low levels of parasitemia [...] increase is proportional to the concentration of hemozoin, with a detection limit of 0.85 ng mL(-1). Malaria parasites in human blood can be detected down to 10 infected red blood cells muL(-1). The assay could
High cure rates and tolerability of artesunate-amodiaquine and dihydroartemisinin-piperaquine for the treatment of uncomplicated falciparum malaria in... (Publications)
BACKGROUND: The Tanzanian National Malaria Control Programme (NMCP) and its partners have been implementing regular therapeutic efficacy studies (TES) to monitor the performance of different drugs used [...] mefantrine, which is the first-line anti-malarial for the treatment of uncomplicated falciparum malaria. Data on the performance of other artemisinin-based combinations is urgently needed to support timely [...] parasitological responses of ASAQ and DP for directly observed treatment of...
Modelling the relationship between malaria prevalence as a measure of transmission and mortality across age groups (Publications)
prevalence has been used widely as a measure of malaria transmission, especially in malaria endemic areas. However, its contribution and relationship to malaria mortality across different age groups has not [...] all-cause and malaria-specific mortality. CONCLUSION: Malaria parasitaemia from cross-sectional surveys was associated with mortality across age groups over 4 to 5 year periods with clinical malaria more strongly [...] surveys from the Kisumu HDSS between 2007 and 2015 were used to...
Videographic analysis of flight behaviours of host-seeking <em>Anopheles arabiensis</em> towards BG-Malaria trap (Publications)
The BG-Malaria trap (BGM) is an adaptation of the well-known BG-Sentinel trap (BGS) with greater trapping efficiencies for anopheline and culicine mosquitoes. Its continued optimization requires greater
Theory of reactive interventions in the elimination and control of malaria (Publications)
in maintaining the disease-free state after achieving malaria elimination (through other interventions). CONCLUSION: RCD alone can eliminate malaria in only a very limited range of settings, where transmission [...] BACKGROUND: Reactive case detection (RCD) is an integral part of many malaria control and elimination programmes and can be conceived of as a way of gradually decreasing transmission. However, it is unclear
Insufficient sensitivity of laser desorption-time of flight mass spectrometry-based detection of hemozoin for malaria screening (Publications)
detection of hemozoin was assessed for its performance characteristics as a rapid screening test for malaria. In spite of good specificity of > 95%, poor sensitivity of 80.2% for microscopically positive samples