Adenosine kinase of <em>T. b. Rhodesiense</em> identified as the putative target of 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine using chemical... (Publications)
show adenosine kinase of T. b. rhodesiense (TbrAK), a key enzyme of the parasite purine salvage pathway which is vital for parasite survival, to be the putative intracellular target of compound 1 using a [...] BACKGROUND: Human African trypanosomiasis (HAT), a major parasitic disease spread in Africa, urgently needs novel targets and new efficacious chemotherapeutic agents. Recently, we discovered that 4-[5
The N'-terminal domain of glyceraldehyde-3-phosphate dehydrogenase of the apicomplexan <em>Plasmodium falciparum</em> mediates GTPase Rab2-dependent... (Publications)
trophozoite stages of both liver and asexual blood stage parasites. During schizogony, pfGAPDH became associated with the periphery of the parasites and eventually accumulated in the apical region of merozoites [...] d that pfGAPDH was found in both the membrane-containing pellet and the supernatant fraction of parasite lysates. In contrast, pfAldolase was only found in the supernatant fraction. A quantitative binding
Improved inhibitors of trypanothione reductase by combination of motifs: synthesis, inhibitory potency, binding mode, and antiprotozoal activities (Publications)
reductase (TR) is an essential enzyme in the trypanothione-based redox metabolism of trypanosomatid parasites. This system is absent in humans and, therefore, offers a promising target for the development of [...] Chagas' disease. Over the past two decades, a variety of nonpeptidic small-molecule ligands of the parasitic enzyme were discovered. A current goal is to decipher the binding mode of these known inhibitors [...] d conjugates exhibit K(ic) values for TR as low as 0.51+/-0.1 muM and...
Comparison of analytical methods for the evaluation of antibody responses against epitopes of polymorphic protein antigens (Publications)
exposed protein antigens of the malaria parasite Plasmodium falciparum frequently harbor multiple dimorphic amino acid positions. These are associated with parasite immune evasion and represent a major obstacle [...] scence analysis (IFA) and Western blotting analysis, the mAbs nevertheless stained all analyzed parasite clones expressing MSP-1(43-53) variant sequences. When used for the evaluation of humoral immune
Anti-trypanosomal activity of Fexinidazole: a new oral nitroimidazole drug candidate for the treatment of sleeping sickness (Publications)
the protozoan parasite Trypanosoma brucei. The compound and its two principal metabolites the sulfoxide and sulfone have been assessed for their ability to kill a range of T. brucei parasite strains in vitro [...] shown trypanocidal activity in vitro in the 0.7 - 3.3 muM (0.2 to 0.9 mug/ml) range against all parasite strains tested. In vivo fexinidazole is orally effective in curing both acute and chronic disease
Adenosine kinase of <em>T. b. Rhodesiense</em> identified as the putative target of 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine using chemical... (Publications)
show adenosine kinase of T. b. rhodesiense (TbrAK), a key enzyme of the parasite purine salvage pathway which is vital for parasite survival, to be the putative intracellular target of compound 1 using a [...] BACKGROUND: Human African trypanosomiasis (HAT), a major parasitic disease spread in Africa, urgently needs novel targets and new efficacious chemotherapeutic agents. Recently, we discovered that 4-[5
Analysis of <em>Plasmodium falciparum</em> infections in a village community in Northern Nigeria: determination of msp2 genotypes and parasitespecific... (Publications)
sm (RFLP). 94.2% of all samples were parasite positive by PCR and over 80% of those had multiple infections. The age distribution of the average number of parasite clones present in P. falciparum infections [...] Mean multiplicity in those 8-10-year-old children was 5.4 clones per carrier. Peak multiplicity and parasite diversity in Nigerian individuals is compared to findings from other study sites in Africa and PNG
High-throughput <em>Plasmodium falciparum hrp2</em> and <em>hrp3 </em>gene deletion typing by digital PCR to monitor malaria rapid diagnostic test... (Publications)
limit of detection was 0.33 parasites/microL. The deletion was reliably detected in mixed infections with wild-type and hrp2-deleted parasites at a density of > 100 parasites/reaction. For a side-by-side
Multiparasitism: a neglected reality on global, regional and local scale (Publications)
communities, particularly in tropical and subtropical areas. Intestinal parasites are the most numerous group, but an array of parasites is located elsewhere than in the human gastrointestinal tract. Although [...] villages highlight the significance of its evaluation for the design of locally adapted and sustainable parasite control and poverty alleviation programmes. We conclude by listing a set of research needs for future
Anti-trypanosomal activity of Fexinidazole: a new oral nitroimidazole drug candidate for the treatment of sleeping sickness (Publications)
the protozoan parasite Trypanosoma brucei. The compound and its two principal metabolites the sulfoxide and sulfone have been assessed for their ability to kill a range of T. brucei parasite strains in vitro [...] shown trypanocidal activity in vitro in the 0.7 - 3.3 muM (0.2 to 0.9 mug/ml) range against all parasite strains tested. In vivo fexinidazole is orally effective in curing both acute and chronic disease
