Parasitological and clinical efficacy of standard treatment regimens against <em>Plasmodium falciparum</em>, <em>P. vivax</em> and <em>P.... (Publications)
quinine+SP. Of the Pv or Pm parasites only one Pv was found to be RII resistant to CQ in the 28-day test. In vitro resistance of Pf to CQ was higher than to AQ (50% versus 27% of 36 parasite samples that grew [...] (PNG). The objective of the study was to assess the rate of clinical treatment failure (TF) and parasite resistance to amodiaquine (AQ), chloroquine (CQ) and quinine+sulfadoxine/pyrimethamine (Q+SP) for
<em>Plasmodium vivax</em> populations are more genetically diverse and less structured than sympatric <em>Plasmodium falciparum</em> populations (Publications)
malaria parasite, Plasmodium vivax, is proving more difficult to control and eliminate than Plasmodium falciparum in areas of co-transmission. Comparisons of the genetic structure of sympatric parasite populations [...] guide malaria control programs. METHODOLOGY/PRINCIPLE FINDINGS: P. vivax isolates representing the parasite populations of four areas on the north coast of Papua New Guinea (PNG) were genotyped using mic
Beyond malaria: causes of fever in outpatient Tanzanian children (Publications)
0% of these infections were radiologically confirmed pneumonia. A systemic bacterial, viral, or parasitic infection other than malaria or typhoid fever was found in 13.3% of children, nasopharyngeal viral [...] A total of 70.5% of the children had viral disease, 22.0% had bacterial disease, and 10.9% had parasitic disease. CONCLUSIONS: These results provide a description of the numerous causes of fever in African [...] settings. Evidence of a viral process was found more commonly than evidence...
Force of infection is key to understanding the epidemiology of <em>Plasmodium falciparum</em> malaria in Papua New Guinean children (Publications)
Genotyping Plasmodium falciparum parasites in longitudinal studies provides a robust approach to estimating force of infection (FOI) in the presence of superinfections. The molecular parameter (mol)FOI [...] hundred sixty-four children 1-3 y of age from Papua New Guinea were followed over 16 mo. Individual parasite clones were tracked longitudinally by genotyping. On average, children acquired 5.9 (SD 9.6) new [...] increased with age, and significantly varied within villages (P = 0.001). The...
The epidemiology of malaria in the Wosera area, East Sepik Province, Papua New Guinea, in preparation for vaccine trials. II. Mortality and morbidity (Publications)
using parasitaemia cut-offs of > or = 1000 Pf parasites/microliter in children aged 1-4 years and adults older than 19 years and of > or = 10,000 parasites/microliter in those aged 5-19 years. Prevalence [...] associated with parasitaemia, and fever plus Plasmodium falciparum (Pf) parasitaemia > or = 10,000 parasites/microliters blood were 5%, 4.1% and 1.5%, respectively. The corresponding figures for adults were
Ecological drivers of <em>Mansonella perstans</em> infection in Uganda and patterns of co-endemicity with lymphatic filariasis and malaria (Publications)
BACKGROUND: Mansonella perstans is a widespread, but relatively unknown human filarial parasite transmitted by Culicoides biting midges. Although it is found in many parts of sub-Saharan Africa, only few [...] epidemiology, and health consequences. Hence, knowledge about ecological drivers of the vector and parasite distribution, integral to develop spatially explicit models for disease prevention, control, and [...] results provide new insight about the ecologic preferences of this otherwise...
Epiisopilosine alkaloid has activity against <em>Schistosoma mansoni</em> in mice without acute toxicity (Publications)
Schistosomiasis is a disease caused by parasites of the genus Schistosoma, currently affecting more than 200 million people. Among the various species of this parasite that infect humans, S. mansoni is the [...] Pharmacological treatment is limited to the use of a single drug, praziquantel (PZQ), despite reports of parasite resistance and low efficacy. It is therefore necessary to investigate new potential schistosomicidal
New myxobacteria of the <em>Myxococcaceae</em> clade produce angiolams with antiparasitic activities (Publications)
options against protozoal parasitic infections, like malaria, is much more severe, especially for the treatment of so-called neglected tropical diseases. The detection of anti-parasitic bioactivities of angiolams [...] its biosynthesis display the opportunities of the angiolam scaffold on route to treat protozoal parasitic infections as well as possible ways to increase the production of derivatives with improved bio
Evaluation of malaria rapid diagnostic test performance and <em>pfhrp2</em> deletion in Tanzania school surveys, 2017 (Publications)
malaria rapid diagnostic tests (mRDTs) in surveys conducted within primary schools to detect malaria parasites. However, mRDTs based on the detection of histidine-rich protein 2 (HRP2) are known to yield fa [...] Plasmodium falciparum hrp2/3 gene deletion. We evaluated diagnostic performance of an HRP2 and pan-parasite lactate dehydrogenase (HRP2/pLDH) mRDT against polymerase chain reaction (PCR) for detection of [...] tive but PCR-positive) were screened for pfhrp2/3 deletion by real-time PCR....
Development and evaluation of PlasmoPod: a cartridge-based nucleic acid amplification test for rapid malaria diagnosis and surveillance (Publications)
ed nucleic acid amplification test for rapid, sensitive, and quantitative detection of malaria parasites. PlasmoPod is based on reverse-transcription quantitative polymerase chain reaction (RT-qPCR) of [...] minutes. Our analytical performance evaluation indicates that a limit-of-detection as low as 0.02 parasites/μL can be achieved and no cross-reactivity with other pathogens common in malaria endemic regions [...] regions was observed. In a cohort of 102 asymptomatic individuals from Bioko...
