Anti-malarial ozonides OZ439 and OZ609 tested at clinically relevant compound exposure parameters in a novel ring-stage survival assay (Publications)
BACKGROUND: Drug efficacy against kelch 13 mutant malaria parasites can be determined in vitro with the ring-stage survival assay (RSA). The conventional assay protocol reflects the exposure profile of [...] I(R539T). CONCLUSION: If the in vitro RSA does indeed predict the potency of compounds against parasites with increased tolerance to artemisinin and its derivatives, then the herein presented data suggest
Identification of an <em>Onchocerca volvulus</em> cDNA encoding a low-molecular-weight antigen uniquely recognized by onchocerciasis patient sera (Publications)
The primary structure of an immunodominant antigen of the filarial parasite, Onchocerca volvulus was deduced from cDNA sequence analysis. Using affinity-purified antibody from onchocerciasis patients from [...] sera from onchocerciasis patients, and not by other sera from patients infected by other filarial parasites, it may prove to be an especially valuable tool for improving the specific diagnosis of onchocerciasis
Inhibition of <em>Plasmodium falciparum</em> Hsp90 contributes to the antimalarial activities of aminoalcohol-carbazoles (Publications)
Malaria caused by the protozoan parasite Plasmodium falciparum (Pf) remains a major public health problem throughout the developing world. One molecular target that should receive more attention is the [...] chaperone Hsp90. It is essential and highly conserved in all eukaryotes, including in protozoan parasites. We have identified an amino-alcohol carbazole (N-CBZ) as a PfHsp90-selective inhibitor by virtually
Three cases of intestinal capillariasis in Lao People's Democratic Republic (Publications)
Capillaria philippinensis is a rare zoonotic intestinal parasite that emerged in the 1960s. The outcome of intestinal capillariasis may be fatal if untreated in due time. We report three cases of intestinal [...] were seen several times in different hospitals before the diagnosis was made. All had concurrent parasite infections: Giardia lamblia, Entamoeba histolytica, Strongyloides stercoralis, Opisthorchis viverrini
Human antibodies to recombinant protein constructs of <em>Plasmodium falciparum</em> Apical Membrane Antigen 1 (AMA1) and their associations with... (Publications)
age and antibody reactivity to whole parasite (schizont) extract. However, these protective associations of antibodies were only seen among subjects that were parasite slide positive at the time of pre-season
1,2-substituted 4-(1H)-quinolones: synthesis, antimalarial and antitrypanosomal activities in vitro (Publications)
cytotoxicity against L-6 cells in vitro. Furthermore, selectivity indices were also determined for both parasites. All compounds tested showed antimalarial activity at low micromolar concentrations, with varied [...] active against P. falciparum, with an IC50 value of 90 nM and good selectivity for the malarial parasite compared to the L-6 cells. Compound 10a, on the other hand, showed a strong antitrypanosomal effect
Discovery and characterization of potent, efficacious, orally available antimalarial plasmepsin X inhibitors and preclinical safety assessment of... (Publications)
Plasmepsin X (PMX) is an essential aspartyl protease controlling malaria parasite egress and invasion of erythrocytes, development of functional liver merozoites (prophylactic activity), and blocking [...] predicted human t1/2. UCB7362 is estimated to achieve 9log10 unit reduction in asexual blood-stage parasites with once-daily dosing of 50 mg for 7 days. This work demonstrates the potential to deliver PMX
A <em>Plasmodium </em>membrane receptor platform integrates cues for egress and invasion in blood forms and activation of transmission stages (Publications)
Critical events in the life cycle of malaria-causing parasites depend on cyclic guanosine monophosphate homeostasis by guanylyl cyclases (GCs) and phosphodiesterases, including merozoite egress or invasion [...] membrane receptor platform that senses signals triggering processes specific to an intracellular parasitic lifestyle, including host cell egress and invasion to ensure intraerythrocytic amplification and
Novel antimalarial tetrazoles and amides active against the hemoglobin degradation pathway in <em>Plasmodium falciparum</em> (Publications)
Assaying parasites with resistance to antimalarials led us to test whether the series had a similar mechanism of action to chloroquine (CQ). Treatment of synchronized Plasmodium falciparum parasites with active
Complement factor D, albumin, and immunoglobulin G anti-band 3 protein antibodies mimic serum in promoting rosetting of malaria-infected red blood... (Publications)
red blood cells (parasitized RBC [pRBC]) with uninfected RBC has been associated in many studies with malaria morbidity and is one form of cytoadherence observed with malarial parasites. Rosetting is serum
