Risk of intestinal parasitic infections in people with different exposures to wastewater and fecal sludge in Kampala, Uganda: a cross-rectional study (Publications)
infection with intestinal parasites and self-reported health outcomes, stratified by population group. PRINCIPAL FINDINGS: The highest point-prevalence of intestinal parasite infections was found in urban [...] low- and middle-income countries. We assessed the point-prevalence and risk factors of intestinal parasite infections in people with different exposures to wastewater and fecal sludge in Kampala, Uganda [...] was administered to determine self-reported signs and symptoms, and risk factors...
Absolute quantification of the host-to-parasite DNA ratio in <em>Theileria parva</em>-infected lymphocyte cell lines (Publications)
quantitative PCR (qPCR) in which recombinant plasmids containing single copy genes specific to the parasite (apical membrane antigen 1 gene, ama1) or the host (hypoxanthine phosphoribosyltransferase 1, hprt1) [...] and 3% of the total DNA samples extracted from these lines. This absolute quantification assay of parasite and host genome copy number in a sample provides a simple and reliable method of assessing T. parva [...] parva load in infected bovine lymphocytes, and is accurate over a wide...
Identification of new human malaria parasite <em>Plasmodium falciparum</em> dihydroorotate dehydrogenase inhibitors by pharmacophore and... (Publications)
species-selectivity for PfDHODH over human DHODH and inhibited parasite growth with an IC50 of 26 muM. In addition to this, 13 compounds inhibited parasite growth with IC50 values of
The prevalence and diversity of intestinal parasitic infections in humans and domestic animals in a rural Cambodian village (Publications)
In Cambodia, intestinal parasitic infections are prevalent in humans and particularly in children. Yet, information on potentially zoonotic parasites in animal reservoir hosts is lacking. In May 2012, [...] al studies will aid characterisation of parasite species and genotypes and allow further insight into the potential for zoonotic cross transmission of parasites in this community. [...] used to confirm hookworm, Ascaris spp., Giardia spp. and Blastocystis spp. Major gastrointestinal parasitic...
Integrated cytokine and metabolic analysis of pathological responses to parasite exposure in rodents (Publications)
Parasitic infections cause a myriad of responses in their mammalian hosts, on immune as well as on metabolic level. A multiplex panel of cytokines and metabolites derived from four parasite-rodent models [...] characterize the urine and plasma metabolite profiles in infected and noninfected animals. Each parasite generated a unique metabolic signature in the host. Plasma cytokine concentrations were obtained [...] signature which is associated with inflammation and to determine specific...
Estimation of infection and recovery rates for highly polymorphic parasites when detectability is imperfect, using hidden Markov models (Publications)
model is proposed for estimating parasitic infection dynamics for highly polymorphic parasites when detectability of the parasite using standard tests is imperfect. The parasite dynamics are modelled as a [...] detection or failure to detect a parasitic genotype. This is assumed to be conditionally independent given the hidden process, that is, the underlying true presence of the parasite, which evolves according to [...] host and bed net use as covariates. This analysis confirmed that the...
