A novel pyrazolopyridine with<em> in vivo</em> activity in <em>Plasmodium berghei</em>- and <em>Plasmodium falciparum</em>-infected mouse models from... (Publications)
efficacy and pharmacokinetics profiles. The lead compounds also proved to be very potent in the parasite liver and gametocyte stages, which makes them of high interest
Amplicon deep sequencing improves <em>Plasmodium falciparum</em> genotyping in clinical trials of antimalarial drugs (Publications)
trials monitoring malaria drug resistance require genotyping of recurrent Plasmodium falciparum parasites to distinguish between treatment failure and new infection occurring during the trial follow up [...] ymorphic markers. AmpSeq was highly reproducible with consistent quantification of co-infecting parasite clones within a host. Outcomes of the three best-performing markers, cpmp, cpp and ama1-D3, agreed
Molecular epidemiology and seroprevalence in asymptomatic <em>Plasmodium falciparum</em> infections of Malagasy pregnant women in the highlands (Publications)
highlands, 67.4% of the women presented antibodies against Plasmodium falciparum and 9.2% were carrying parasites, at the coast 95.6% and 14.8%, respectively. In the mean, 1.2 clones were detected in infected pregnant [...] CONCLUSION: Immunity is triggered in Malagasy highland populations when they are infected with malaria parasites, but these populations could also serve as a reservoir for epidemics.
Effect of insecticide-treated bed nets on the dynamics of multiple <em>Plasmodium falciparum</em> infections (Publications)
intervals of one month indicated that the average duration of infections, allowing for undetected parasite genotypes, was 73 d in those aged < 18 months and 160 d in children aged > or = 18 months, consistent [...] (SE = 6%) compared with 41% (SE = 6%) in older children. Conversely, the rate of appearance of new parasite genotypes was higher in children < 18 months of age than in older children, but this partly reflected
Circulating Anodic Antigen (CAA): a highly sensitive diagnostic biomarker to detect active <em>Schistosoma </em>infections-improvement and use during... (Publications)
larger SCORE studies. For example, in Zanzibar, we demonstrated that urine filtration, the standard parasite egg detection diagnostic test for S. haematobium, greatly underestimated prevalence in low-prevalence [...] antigen (CAA) assay provided critical information about the limitations of the stool-based Kato-Katz parasite egg-detection assay for S. mansoni in low-prevalence settings. Other SCORE-supported CAA work d
Use of poly(amidoamine) drug conjugates for the delivery of antimalarials to <em>Plasmodium</em> (Publications)
deliver drugs to Plasmodium-infected red blood cells (pRBCs) in order to limit the appearance of parasite resistance. Here, the poly(amidoamines) AGMA1 and ISA23 have been explored for the delivery of [...] preferential binding to and internalization into pRBCs vs. RBCs, and subcellular targeting to the parasite itself in widely diverging species such as P. falciparum and P. yoelii, infecting humans and mice
Toward the development of dual-targeted glyceraldehyde-3-phosphate dehydrogenase/trypanothione reductase inhibitors against <em>Trypanosoma... (Publications)
characterized for their dual-target antitrypanosomal profile, both in enzyme assays and in in vitro parasite cultures. The merged derivative 2-{[3-(3-dimethylaminopropoxy)-2-oxo-2H-chromen-7-yl]oxy}anthracene-1 [...] -yl]phenoxy}anthracene-1,4-dion e (compound 6) displayed a remarkable EC50 value for T. brucei parasites (0.026 muM) combined with a very low cytotoxicity toward mammalian L6 cells (7.95 muM). This promising
Anti-trypanosomatid elemanolide sesquiterpene lactones from<em> Vernonia lasiopus </em>O. Hoffm (Publications)
developed regions of sub-Saharan Africa and is caused by the kinetoplastid "protozoan" parasite Trypanosoma brucei. The parasites are transmitted to humans through bites of infected tsetse flies of the genus Glossina
Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests (Publications)
detail. RESULTS: Over 30 pathogens may cause persistent digestive disorders. Bacteria, viruses and parasites are important aetiologic agents of acute and long-lasting symptomatologies. An integrated approach [...] for toxin, antigen and antibody detection, is required for accurate diagnosis of bacteria and parasites. Molecular techniques are essential for sensitive diagnosis of many viruses, bacteria and intestinal
Metabolic alterations in the hamster co-infected with <em>Schistosoma japonicum</em> and <em>Necator americanus</em> (Publications)
d a combination of the altered metabolite profiles induced by single infections with these two parasites. Consistent differences in metabolic profiles between the co-infected and non-infected control hamsters [...] provide a first step towards understanding the metabolic response of an animal host to multiple parasitic infections
