Molecular markers of in vivo <em>Plasmodium vivax</em> resistance to amodiaquine plus sulfadoxine-pyrimethamine: mutations in pvdhfr and pvmdr1 (Publications)
amodiaquine (AQ) plus SP in Papua New Guinea (PNG). We then investigated the association between parasite genotype and treatment response. Results. @nbsp; The treatment failure (TF) rate reached 13%. P [...] in TF rates between sites was reflected in the genetic drug-resistance profile of the respective parasites. Conclusions. @nbsp; The present study identified a new molecular marker in pvmdr1 that is associated
Antigenicity and immunogenicity of a novel <em>Plasmodium vivax</em> circumsporozoite derived synthetic vaccine construct (Publications)
domains involved in parasite invasion of host cells. The protein amino (N-terminal) flank has a cleavage region (region I), essential for proteolytic processing prior to parasite invasion of liver cells
Therapeutic development to accelerate malaria control through intentional intervention layering (Publications)
products requires monitoring health outcomes that reflect the novel product's targeted stage of the parasite life cycle, in addition to all-infection and prevalence-based outcomes. Second, what public health [...] protection. Specifically, this means designing products with components that target parts of the parasite life cycle beyond the scope of existing therapeutics, and better addressing populations and settings
The genomes of three stocks comprising the most widely utilized live sporozoite <em>Theileria parva</em> vaccine exhibit very different degrees and... (Publications)
BACKGROUND: There are no commercially available vaccines against human protozoan parasitic diseases, despite the success of vaccination-induced long-term protection against infectious diseases. East Coast [...] protection against infections in the field, our results suggest that whole-organism vaccines against parasitic diseases can be highly efficacious despite considerable genome-wide differences relative to the
Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis (Publications)
diagnostics, an early and rapid diagnosis is required. So far, classic diagnostic tools such as parasite microscopy or serology lack considerable sensitivity at this early stage of Schistosoma infection [...] 92%). In contrast, sensitivity of serology (enzyme immunoassay and/or immunofluorescence assay) or parasite microscopy was only 70% and 24%, respectively. CONCLUSION: For the early diagnosis of acute sch
<em>In vitro</em> antischistosomal activity of <em>Bridelia ferruginea, Clausena anisata, Khaya senegalensis, </em>and <em>Vernonia amygdalina</em> (Publications)
BACKGROUND: Schistosomiasis is caused by parasitic flatworms and the disease is endemic to most countries in sub-Saharan Africa including Ghana. The current therapeutic agent for managing this disease [...] exposed to the extracts diluted in the RPMI medium. The assay was performed in a 24-well plate. The parasitic worms were examined using an inverted optical microscope. RESULTS: At 100 μg/mL and 50 μg/mL, all
Investigating the drivers of the spatio-temporal patterns of genetic differences between <em>Plasmodium falciparum</em> malaria infections in Kilifi... (Publications)
trials to assess the interventions. A previous analysis of 1602 genotyped Plasmodium falciparum parasites in Kilifi, Kenya collected over 12 years found an interaction between time and geographic distance: [...] clearance were included. We fit the model to the observed numbers of SNP differences between pairs of parasite genotypes. The patterns observed in the empiric data could be reproduced. Although we cannot rule
Association between gastrointestinal tract infections and glycated hemoglobin in school children of poor neighborhoods in Port Elizabeth, South Africa (Publications)
tract infections) and non-communicable diseases (e.g., diabetes) being common. For instance, chronic parasite infections lead to altered immune regulatory networks, anemia, malnutrition, and diarrhea with an [...] children in Port Elizabeth. Stool and urine samples were collected to assess infection status with parasitic worms (e.g., Ascaris lumbricoides, Enterobius vermicularis, and Trichuris trichiura), intestinal
High proportions of asymptomatic and submicroscopic <em>Plasmodium vivax </em>infections in a peri-urban area of low transmission in the Brazilian... (Publications)
including all inhabitants in a peri-urban area of Manaus, western Amazonas State, Brazil. Malaria parasites were detected by light microscopy (LM) and qPCR. Sexual stages of Plasmodium spp. were detected [...] P. vivax infections, of which 42.9% (30/70) of infections were gametocyte positive. The P. vivax parasite density was associated with gametocyte carriage (P < 0.001). Sixty-seven percent of P. vivax infected
Impact and cost-effectiveness of snail control to achieve disease control targets for schistosomiasis (Publications)
Schistosomiasis is a parasitic disease that affects over 240 million people globally. To improve population-level disease control, there is growing interest in adding chemical-based snail control interventions [...] interventions to interrupt the lifecycle of Schistosoma in its snail host to reduce parasite transmission. However, this approach is not widely implemented, and given environmental concerns, the optimal conditions
