Whole blood transcriptome changes following controlled human malaria infection in malaria pre-exposed volunteers correlate with parasite prepatent... (Publications)
signaling and erythrocyte development. Our study showed that in malaria pre-exposed volunteers, parasite prepatent period in each individual is linked to magnitude and timing of early gene expression changes
Challenges of DHS and MIS to capture the entire pattern of malaria parasite risk and intervention effects in countries with different ecological... (Publications)
peak transmission season. This study compares estimates of the geographical distribution of malaria parasite risk and of the effects of interventions obtained from the DHS and MIS survey data. METHODS: Bayesian [...] geostatistical models were applied on DHS and MIS data to obtain georeferenced estimates of the malaria parasite prevalence and to assess the effects of interventions. Climatic predictors were retrieved from satellite [...] climatic predictors and indicators of malaria...
The mitochondrial genomes of the zoonotic canine filarial parasites<em> Dirofilaria (Nochtiella) repens</em> and <em>Candidatus</em> Dirofilaria... (Publications)
hongkongensis, has been reported. The present study aimed to compare mitochondrial genomes from these parasites and to obtain population genetic information. METHODS AND FINDINGS: Complete mitochondrial genomes
Influence of parasite density and sample storage time on the reliability of <em>Entamoeba histolytica</em>-specific PCR from formalin-fixed and... (Publications)
sections and in 5 samples without visible parasites in neighboring sections. PCR was negative in all specimens that were older than 3 decades. Low parasite counts and sample ages older than 20 years were [...] amebiasis. Specimens had been collected during the previous 4 decades. Association of sample age, parasite density, and reliability of PCR was analyzed. E. histolytica PCR was positive in 20 of 34 biopsies [...] paraffin-embedded tissue samples that are younger than 2 decades and that...
The <em>Plasmodium falciparum</em> histone methyltransferase PfSET10 is dispensable for the regulation of antigenic variation and gene expression in... (Publications)
expression during asexual parasite development and gametocytogenesis. Interestingly, however, PfSET10 shows different subnuclear localization patterns in asexual and sexual stage parasites and female-specific [...] for blood-stage parasite viability, indicating PfSET10 may be important for life cycle progression in the mosquito vector or during liver stage development.IMPORTANCEThe malaria parasite Plasmodium falciparum [...] The malaria parasite Plasmodium falciparum employs antigenic variation...
The influence of malaria control interventions and climate variability on changes in the geographical distribution of parasite prevalence in Kenya... (Publications)
plateau in recent years. This study estimated changes in the geographical distribution of malaria parasite risk in the country between the years 2015 and 2020, and quantified the contribution of malaria
3-Benzylmenadiones and their heteroaromatic analogues target the apicoplast of apicomplexa parasites: synthesis and bioimaging studies (Publications)
The apicoplast is an essential organelle for the viability of apicomplexan parasites Plasmodium falciparum or Toxoplasma gondii, which has been proposed as a suitable drug target for the development of [...] the clicked products localize within, or in the vicinity of, the apicoplast of both Apicomplexa parasites. In P. falciparum, the fluorescence signal was also associated with the mitochondrion, suggesting [...] plasmodione and related analogues are potentially associated with these two...
Identification of new human malaria parasite <em>Plasmodium falciparum</em> dihydroorotate dehydrogenase inhibitors by pharmacophore and... (Publications)
species-selectivity for PfDHODH over human DHODH and inhibited parasite growth with an IC50 of 26 muM. In addition to this, 13 compounds inhibited parasite growth with IC50 values of
Absolute quantification of the host-to-parasite DNA ratio in <em>Theileria parva</em>-infected lymphocyte cell lines (Publications)
quantitative PCR (qPCR) in which recombinant plasmids containing single copy genes specific to the parasite (apical membrane antigen 1 gene, ama1) or the host (hypoxanthine phosphoribosyltransferase 1, hprt1) [...] and 3% of the total DNA samples extracted from these lines. This absolute quantification assay of parasite and host genome copy number in a sample provides a simple and reliable method of assessing T. parva [...] parva load in infected bovine lymphocytes, and is accurate over a wide...
