Micro-epidemiological structuring of <em>Plasmodium falciparum</em> parasite populations in regions with varying transmission intensities in Africa (Publications)
and homogeneous population of parasites. Recent models include spatial heterogeneity and variably mixed populations. However, there are few empiric estimates of parasite mixing with which to parametize [...] extent of parasite mixing, and use Principal Component Analysis (PCA) and linear regression to examine the relationship between genetic relatedness and relatedness in space and time for parasite pairs. Results: [...] show that there are no discrete geographically restricted parasite...
Gastrointestinal parasites and bacteria in free-living South American sea lions (<em>Otaria flavescens</em>) in Chilean Comau Fjord and new host... (Publications)
with five different gastrointestinal parasite genera. In addition, bacterial cultures showed presence of at least 28 different bacterial genera. Referring to parasites, protozoan, and metazoan species were [...] Present study aimed to characterize gastrointestinal parasites and culturable bacteria from free-living South American sea lions (Otaria flavescens) inhabiting waters of Comau Fjord, Patagonia, Chile. [...] onotic potential and/or pathogenic impact for these marine mammals. As such, four...
Intravital imaging of host-parasite interactions in skin and adipose tissues (Publications)
in living animals in real time. This method has enabled key advances in our understanding of host-parasite interactions under physiological conditions. A combination of genetics, microscopy techniques and [...] findings achieved by intravital microscopy of the skin and adipose tissues upon infection with various parasites and we present a view into possible future applications of this method.
Evaluation of 4-amino 2-anilinoquinazolines against <em>Plasmodium</em> and other apicomplexan parasites<em> in vitro </em>and in a<em> P.... (Publications)
array of P. falciparum parasites resistant to clinically available anti-malarial compounds although slightly less so than the drug sensitive 3D7 parasite line. The apicomplexan parasites Toxoplasma gondii, [...] for activity against the most lethal malaria parasite of humans, Plasmodium falciparum, were evaluated for activity against other human Plasmodium parasites and related apicomplexans that infect humans [...] yet to be discovered target of these compounds is absent or highly divergent in...
The three <em>Plasmodium falciparum </em>Aurora-related kinases display distinct temporal and spatial associations with mitotic structures in asexual... (Publications)
been reported essential for parasite proliferation, but their roles in regulating schizogony have not yet been explored in great detail. Here, we engineered transgenic parasite lines expressing GFP-tagged [...] kinases are crucial regulators of mitotic cell cycle progression in eukaryotes. The protozoan malaria parasite Plasmodium falciparum replicates via schizogony, a specialised mode of cell division characterized [...] Aurora-related kinases in mitotic cell division of Plasmodium falciparum...
Screening of malaria infections in human blood samples with varying parasite densities and anaemic conditions using AI-powered mid-infrared... (Publications)
mid-infrared spectra from dried blood spots to accurately detect malaria infections across varying parasite densities and anaemic conditions. METHODS: Plasmodium falciparum strains NF54 and FCR3 were cultured [...] RESULTS: The AI classifiers attained over 90% accuracy in detecting malaria infections as low as one parasite per microlitre of blood, a sensitivity unattainable by conventional RDTs and microscopy. These [...] cost-effective method for malaria screening, consistently performing well...
Seasonal malaria chemoprevention and the spread of <em>Plasmodium falciparum </em>quintuple-mutant parasites resistant to sulfadoxine-pyrimethamine: a... (Publications)
evaluated the spread of quintuple-mutant parasites and the clinical consequences. METHODS: We used an individual-based malaria transmission model with explicit parasite dynamics and drug pharmacological models [...] cases in children younger than 5 years in Africa's Sahel region. However, Plasmodium falciparum parasites partially resistant to sulfadoxine-pyrimethamine (with quintuple mutations) potentially threaten [...] chemoprevention promoted mutant spread. When SMC was implemented in a...
Spatial distribution of<em> Falciparum</em> malaria infections in Zanzibar: implications for focal drug administration strategies targeting... (Publications)
Plasmodium falciparum infections to compare simulated effects of these strategies on reducing the parasite reservoir in a pre-elimination setting. Methods.: P. falciparum rapid diagnostic tests (RDTs) and
