The origins of malaria artemisinin resistance defined by a genetic and transcriptomic background (Publications)
ion linkages in the eastern GMS parasites and 4537 in the western. The minimal overlap between them suggests differential gene regulatory networks facilitating parasite adaptations to their unique host [...] The predisposition of parasites acquiring artemisinin resistance still remains unclear beyond the mutations in Pfk13 gene and modulation of the unfolded protein response pathway. To explore the chain of [...] from TRACI-study integrating TWAS, GWAS, and eQTL analyses. We find the majority of...
The origins of malaria artemisinin resistance defined by a genetic and transcriptomic background (Publications)
ion linkages in the eastern GMS parasites and 4537 in the western. The minimal overlap between them suggests differential gene regulatory networks facilitating parasite adaptations to their unique host [...] The predisposition of parasites acquiring artemisinin resistance still remains unclear beyond the mutations in Pfk13 gene and modulation of the unfolded protein response pathway. To explore the chain of [...] from TRACI-study integrating TWAS, GWAS, and eQTL analyses. We find the majority of...
A point mutation in codon 76 of <em>pfcrt </em>of <em>P. falciparum </em>is positively selected for by chloroquine treatment in Tanzania (Publications)
Both values exceeded by far the observed frequency of 14% of recrudescent parasites as calculated on the whole analysed parasite population taking multiple infections into account. Although neither mutant [...] CQ resistance in the natural parasite population. All wild-type pfcrt(76) alleles were eliminated before day 3, after the onset of CQ treatment and no recrudescent parasite with the wild-type allele was [...] the number of concurrent infections was also determined. The rate of children...
Non-invasive monitoring of drug action: a new live <em>in vitro</em> assay design for Chagas' disease drug discovery (Publications)
(eGFP)-expressing parasite line and live high-content imaging to monitor the growth of T. cruzi amastigotes in mouse embryonic fibroblasts. A novel assay design allowed us to follow parasite numbers over 6 [...] first decreased over the time of drug exposure, and then leveled off. However, we observed that parasite numbers could vary, even in the untreated controls, and at different sites in the same well, which [...] which caused variability in the EC50 values. To overcome this, we established...
Antigens released at schizont burst stimulate <em>Plasmodium falciparum</em>-specific CD4+ T cells from non-exposed donors: potential for... (Publications)
non-exposed individuals can be stimulated by malaria parasites. Here, we show that nine from 11 blood samples, naturally infected with malaria parasites, could stimulate proliferation of a malaria-specific [...] TCR V beta genes are expressed during the T cell response of naive donors to malaria parasites, processing of parasite antigens is blocked by chloroquine and monensin, and activation of 36 of 41 malaria-specific [...] clones tested was restricted by defined MHC class II allelic antigens,...
Antigens released at schizont burst stimulate <em>Plasmodium falciparum</em>-specific CD4+ T cells from non-exposed donors: potential for... (Publications)
non-exposed individuals can be stimulated by malaria parasites. Here, we show that nine from 11 blood samples, naturally infected with malaria parasites, could stimulate proliferation of a malaria-specific [...] TCR V beta genes are expressed during the T cell response of naive donors to malaria parasites, processing of parasite antigens is blocked by chloroquine and monensin, and activation of 36 of 41 malaria-specific [...] clones tested was restricted by defined MHC class II allelic antigens,...
Non-invasive monitoring of drug action: a new live <em>in vitro</em> assay design for Chagas' disease drug discovery (Publications)
(eGFP)-expressing parasite line and live high-content imaging to monitor the growth of T. cruzi amastigotes in mouse embryonic fibroblasts. A novel assay design allowed us to follow parasite numbers over 6 [...] first decreased over the time of drug exposure, and then leveled off. However, we observed that parasite numbers could vary, even in the untreated controls, and at different sites in the same well, which [...] which caused variability in the EC50 values. To overcome this, we established...
A point mutation in codon 76 of <em>pfcrt </em>of <em>P. falciparum </em>is positively selected for by chloroquine treatment in Tanzania (Publications)
Both values exceeded by far the observed frequency of 14% of recrudescent parasites as calculated on the whole analysed parasite population taking multiple infections into account. Although neither mutant [...] CQ resistance in the natural parasite population. All wild-type pfcrt(76) alleles were eliminated before day 3, after the onset of CQ treatment and no recrudescent parasite with the wild-type allele was [...] the number of concurrent infections was also determined. The rate of children...
Assessing the impact of next-generation rapid diagnostic tests on <em>Plasmodium falciparum</em> malaria elimination strategies (Publications)
infectious to mosquitoes. We estimate the relationship between parasite density and onward infectivity using sensitive quantitative parasite diagnostics and mosquito feeding assays from Burkina Faso. We [...] how often each individual is bitten) whereas a test with a limit of 20 parasites per microlitre would detect 83% and 2 parasites per microlitre would detect 95% of the infectious reservoir. Using mathematical [...] that increasing the diagnostic sensitivity from 200 parasites per microlitre...
Assessing the impact of next-generation rapid diagnostic tests on <em>Plasmodium falciparum</em> malaria elimination strategies (Publications)
infectious to mosquitoes. We estimate the relationship between parasite density and onward infectivity using sensitive quantitative parasite diagnostics and mosquito feeding assays from Burkina Faso. We [...] how often each individual is bitten) whereas a test with a limit of 20 parasites per microlitre would detect 83% and 2 parasites per microlitre would detect 95% of the infectious reservoir. Using mathematical [...] that increasing the diagnostic sensitivity from 200 parasites per microlitre...
