Ensuring transmission through dynamic host environments: host-pathogen interactions in <em>Plasmodium </em>sexual development (Publications)
vaccines. The identification of parasite factors that alter sexual differentiation, including extracellular vesicles and a master transcriptional regulator, suggest that parasites make epigenetically controlled [...] especially host cell remodeling and sequestration in the bone marrow, highlight open questions regarding parasite homing to the tissue, transmigration across the vascular endothelium, and maturation in the parenchyma [...] parenchyma. Novel molecular and translational tools will...
The shape of the iceberg: quantification of submicroscopic <em>Plasmodium falciparum</em> and<em> Plasmodium vivax </em>parasitaemia and... (Publications)
by microscopy in 881 slides (0.7%), 107 of 845 blood samples (12.7%) were parasite positive by (DNA-based) qPCR. qPCR parasite prevalence between sites and surveys ranged from 3.8 to 19.0% for P. falciparum [...] density of P. falciparum infections (n = 85) was 24.4 parasites/microL (IQR 18.0-34.0) and the median density of P. vivax infections (n = 28) was 16.4 parasites/microL (IQR 8.8-55.1). Gametocyte densities by [...] submicroscopic parasite densities and concurrent gametocyte densities....
The shape of the iceberg: quantification of submicroscopic <em>Plasmodium falciparum</em> and<em> Plasmodium vivax </em>parasitaemia and... (Publications)
by microscopy in 881 slides (0.7%), 107 of 845 blood samples (12.7%) were parasite positive by (DNA-based) qPCR. qPCR parasite prevalence between sites and surveys ranged from 3.8 to 19.0% for P. falciparum [...] density of P. falciparum infections (n = 85) was 24.4 parasites/microL (IQR 18.0-34.0) and the median density of P. vivax infections (n = 28) was 16.4 parasites/microL (IQR 8.8-55.1). Gametocyte densities by [...] submicroscopic parasite densities and concurrent gametocyte densities....
Clonal expansion of artemisinin-resistant <em>Plasmodium falciparum</em> in Uganda is associated with substandard treatment practices (Publications)
therapy. Parasite editing and phenotyping were performed to assess the impact of mutations on parasites resistance while whole-genome sequencing was used to specify the phylogeny of resistant parasites. Findings: [...] whom 44% were treated with an artemisinin monotherapy. Parasite editing and phenotyping confirmed reduced susceptibility to artemisinin in parasites with C469Y mutation while phylogenetic analyses demonstrated [...] artesunate monotherapies are associated with the selection of an...
[Preprint] Clonal expansion of artemisinin-resistant <em>Plasmodium falciparum</em> in Uganda is associated with substandard treatment practices (Publications)
therapy. Parasite editing and phenotyping were performed to assess the impact of mutations on parasites resistance while whole-genome sequencing was used to specify the phylogeny of resistant parasites. Findings: [...] whom 44% were treated with an artemisinin monotherapy. Parasite editing and phenotyping confirmed reduced susceptibility to artemisinin in parasites with C469Y mutation while phylogenetic analyses demonstrated [...] artesunate monotherapies are associated with the selection of an...
An assay to probe <em>Plasmodium falciparum</em> growth, transmission stage formation and early gametocyte development (Publications)
conversion and early gametocyte development in the major human malaria parasite Plasmodium falciparum. Synchronized asexually replicating parasites are induced for gametocyte production by the addition of conditioned [...] proliferation to sexual differentiation initiates the production of the gametocyte, which is the malaria parasite stage required for human-to-mosquito transmission. This protocol describes an assay designed to [...] on-blocking compounds and to further investigate the...
<em>Plasmodium falciparum</em>-infected erythrocytes: agglutination by diverse Kenyan plasma is associated with severe disease and young host age (Publications)
infecting parasites. Previously, when different parasite isolates were tested against a panel of heterologous plasma from Kenyan children, the proportion of plasma that agglutinated the parasites (the ag [...] malaria; and AF decreased significantly with the increasing age of the infected child. We propose that parasites causing severe disease tend to express a subset of VSA variants that are preferentially associated
<em>Plasmodium falciparum</em>-infected erythrocytes: agglutination by diverse Kenyan plasma is associated with severe disease and young host age (Publications)
infecting parasites. Previously, when different parasite isolates were tested against a panel of heterologous plasma from Kenyan children, the proportion of plasma that agglutinated the parasites (the ag [...] malaria; and AF decreased significantly with the increasing age of the infected child. We propose that parasites causing severe disease tend to express a subset of VSA variants that are preferentially associated
An assay to probe <em>Plasmodium falciparum</em> growth, transmission stage formation and early gametocyte development (Publications)
conversion and early gametocyte development in the major human malaria parasite Plasmodium falciparum. Synchronized asexually replicating parasites are induced for gametocyte production by the addition of conditioned [...] proliferation to sexual differentiation initiates the production of the gametocyte, which is the malaria parasite stage required for human-to-mosquito transmission. This protocol describes an assay designed to [...] on-blocking compounds and to further investigate the...
Next generation chemiluminescent probes for antimalarial drug discovery (Publications)
ytic P. falciparum parasites without the need for parasite enrichment, washing, or purification steps. We are convinced that the ultralow detection limit of less than 100 parasites of the presented biosensor [...] Malaria is caused by parasites of the Plasmodium genus and remains one of the most pressing human health problems. The spread of parasites resistant to or partially resistant to single or multiple drugs
