Metabolic characterization of <em>Leishmania major</em> infection in activated and nonactivated macrophages (Publications)
main biochemical process responsible for either nitric oxide mediated parasite killing (classical activation) or amplification of parasite replication (alternative activation), we were interested in a wider [...] state and macrophage activation, whereby significantly higher levels of potential parasite end products were found in parasite exposed samples including succinate, acetate, and alanine, compared to uninfected [...] wider characterization of metabolic events in vitro. We therefore...
From magic bullet to magic bomb: reductive bioactivation of antiparasitic agents (Publications)
describe how a drug kills the parasite inside its human host without harming the host itself. Ehrlich concluded that the drug must have a greater affinity to the parasite than to human cells. Today, the [...] cells. Examples are the enzymes of folate synthesis or of the nonmevalonate pathway in the malaria parasites. However, there are other ways how a drug can kill selectively. Of particular relevance is the specific [...] of a prodrug inside the pathogen but not in the host, as this is how the...
From magic bullet to magic bomb: reductive bioactivation of antiparasitic agents (Publications)
describe how a drug kills the parasite inside its human host without harming the host itself. Ehrlich concluded that the drug must have a greater affinity to the parasite than to human cells. Today, the [...] cells. Examples are the enzymes of folate synthesis or of the nonmevalonate pathway in the malaria parasites. However, there are other ways how a drug can kill selectively. Of particular relevance is the specific [...] of a prodrug inside the pathogen but not in the host, as this is how the...
Next generation chemiluminescent probes for antimalarial drug discovery (Publications)
ytic P. falciparum parasites without the need for parasite enrichment, washing, or purification steps. We are convinced that the ultralow detection limit of less than 100 parasites of the presented biosensor [...] Malaria is caused by parasites of the Plasmodium genus and remains one of the most pressing human health problems. The spread of parasites resistant to or partially resistant to single or multiple drugs
Metabolic characterization of <em>Leishmania major</em> infection in activated and nonactivated macrophages (Publications)
main biochemical process responsible for either nitric oxide mediated parasite killing (classical activation) or amplification of parasite replication (alternative activation), we were interested in a wider [...] state and macrophage activation, whereby significantly higher levels of potential parasite end products were found in parasite exposed samples including succinate, acetate, and alanine, compared to uninfected [...] wider characterization of metabolic events in vitro. We therefore...
Characterization of field malaria isolates and assessment of the impact of resistance profile on the transmission of resistant strains (Projects)
https://www.swisstph.ch/en/projects/project-detail/project/characterization-of-field-malaria-isolates-and-assessment-of-the-impact-of-resistance-profile-on-the-transmission-of-resistant-strains
this has been done using laboratory reared parasites. There is therefore a need for these tools and novel technologies to be evaluated using field malaria parasites, in order to provide a more realistic r [...] settings. We will establish a continuous culture protocol for field malaria parasite isolates and characterize the parasites at a molecular level, including genetic diversity and drug resistance profile [...] will evaluate the effect of insecticides like chlorfenapyr on the development of...
Identification of fast-acting 2,6-disubstituted imidazopyridines that are efficacious in the <em>In Vivo</em> humanized <em>Plasmodium falciparum</em>... (Publications)
asexual blood stage parasites that, together with their in vitro absorption, distribution, metabolism, and excretion (ADME) properties, translated to in vivo efficacy with clearance of parasites in the PfSCID [...] of a chemical series originating from whole-cell phenotypic screening against the human malaria parasite, Plasmodium falciparum, led to the identification of two promising 2,6-disubstituted imidazopyridine
Novel inhibitors of Trypanosoma cruzi dihydrofolate reductase (Publications)
DOCK 3.5. Compounds were then tested against the enzyme and the whole parasite. Compounds were also screened against the related parasite, Trypanosoma brucei [...] need for the development of new drugs to treat Chagas' disease, which is caused by the protozoan parasite Trypanosoma cruzi. The enzyme dihydrofolate reductase (DHFR) has been a very successful drug target
Influences of blood digestion on the development of <em>Plasmodium gallinaceum</em> (Brumpt) in the midgut of <em>Aedes aegypti</em> (L.) (Publications)
action of trypsin-like proteases on the parasites and indicate that plasmodia 0-10 h after blood meal are more sensitive to the enzymes than later stages of the parasites [...] shifted against each other by giving the mosquitoes two consecutive blood meals. In this way the parasites were exposed to an environment where blood digestion was more advanced than in single blood meals
Novel inhibitors of Trypanosoma cruzi dihydrofolate reductase (Publications)
DOCK 3.5. Compounds were then tested against the enzyme and the whole parasite. Compounds were also screened against the related parasite, Trypanosoma brucei [...] need for the development of new drugs to treat Chagas' disease, which is caused by the protozoan parasite Trypanosoma cruzi. The enzyme dihydrofolate reductase (DHFR) has been a very successful drug target
