CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for <em>Plasmodium falciparum </em>malaria transmission research (Publications)
The malaria parasite Plasmodium falciparum replicates inside erythrocytes in the blood of infected humans. During each replication cycle, a small proportion of parasites commits to sexual development and [...] and differentiates into gametocytes, which are essential for parasite transmission via the mosquito vector. Detailed molecular investigation of gametocyte biology and transmission has been hampered by [...] are transmissible by mosquitoes. We also demonstrate that further genetic...
CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for <em>Plasmodium falciparum </em>malaria transmission research (Publications)
The malaria parasite Plasmodium falciparum replicates inside erythrocytes in the blood of infected humans. During each replication cycle, a small proportion of parasites commits to sexual development and [...] and differentiates into gametocytes, which are essential for parasite transmission via the mosquito vector. Detailed molecular investigation of gametocyte biology and transmission has been hampered by [...] are transmissible by mosquitoes. We also demonstrate that further genetic...
Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria (Publications)
outcome of individuals infected with Plasmodium falciparum parasites depends on many factors including host systemic inflammatory responses, parasite sequestration in tissues and vascular dysfunction. Production [...] in turn triggers further endothelial cell activation and parasite sequestration. Inflammatory responses are triggered in part by bioactive parasite products such as hemozoin and infected red blood cell-derived
Laboratories | Research Infrastructure at Swiss TPH (Page)
https://www.swisstph.ch/en/research/research-infrastructure/laboratories
hosts several parasitic cycles that are used for drug development and testing, for example in the field of helminth research or as a source of antigens for diagnostics. Hosting a parasitic cycle includes [...] includes the parasite at all stages of its life, the intermediate and the final host(s). The parasitic cycles we grow depend on ongoing research projects, but we have four permanent helminth cycles, namely [...] new drug and vaccine candidates. A researcher is adding culture medium to...
Molecular detection of <em>Setaria tundra</em> (Nematoda: Filarioidea) and an unidentified filarial species in mosquitoes in Germany (Publications)
conducted a molecular mass screening of mosquitoes for filarial parasites using mitochondrial 12S rRNA-based real-time PCR. RESULTS: No parasites causing disease in humans such as Dirofilaria spp. were detected [...] which could be attributed to mosquito infection with Setaria tundra and a yet unidentified second parasite. Setaria tundra was found to be widespread in southern Germany in various mosquito species, except [...] species was exclusively found in Culex spp. in northern...
Indigenous emergence and spread of <em>kelch13</em> C469Y artemisinin-resistant <em>Plasmodium falciparum</em> in Uganda (Publications)
(RAS) in 2019. K13 polymorphisms were assessed, and parasite editing and phenotyping were performed to assess the impact of mutations on parasite resistance. Whole-genome sequencing was performed, and [...] decades ago. Recent studies in East Africa have reported a gradual increase in kelch13 (k13) mutant parasites associated with reduced artesunate efficacy. As part of the Community Access to Rectal Artesunate [...] on. Genome editing confirmed reduced in vitro susceptibility to artemisinin in...
Diagnostic performance of conventional and ultrasensitive rapid diagnostic tests for malaria in febrile outpatients in Tanzania (Publications)
co-RDT (> 3653 pg /ml) in almost all (99%, 236/239) infections with parasite density above 100 parasites/microl. Below 100 parasites/microl, HRP2 concentration was above the detectable limits by us-RDT [...] (co-RDT) in 3000 children and 515 adults presenting with fever to Tanzanian outpatient clinics. Parasite density by ultra-sensitive qPCR (us-qPCR) and HRP2 concentration by ELISA were measured. Results: [...] malaria diagnosis. In febrile patients, only a small proportion of infections are...
Molecular detection of <em>Setaria tundra</em> (Nematoda: Filarioidea) and an unidentified filarial species in mosquitoes in Germany (Publications)
conducted a molecular mass screening of mosquitoes for filarial parasites using mitochondrial 12S rRNA-based real-time PCR. RESULTS: No parasites causing disease in humans such as Dirofilaria spp. were detected [...] which could be attributed to mosquito infection with Setaria tundra and a yet unidentified second parasite. Setaria tundra was found to be widespread in southern Germany in various mosquito species, except [...] species was exclusively found in Culex spp. in northern...
Diagnostic performance of conventional and ultrasensitive rapid diagnostic tests for malaria in febrile outpatients in Tanzania (Publications)
co-RDT (> 3653 pg /ml) in almost all (99%, 236/239) infections with parasite density above 100 parasites/microl. Below 100 parasites/microl, HRP2 concentration was above the detectable limits by us-RDT [...] (co-RDT) in 3000 children and 515 adults presenting with fever to Tanzanian outpatient clinics. Parasite density by ultra-sensitive qPCR (us-qPCR) and HRP2 concentration by ELISA were measured. Results: [...] malaria diagnosis. In febrile patients, only a small proportion of infections are...
Indigenous emergence and spread of <em>kelch13</em> C469Y artemisinin-resistant <em>Plasmodium falciparum</em> in Uganda (Publications)
(RAS) in 2019. K13 polymorphisms were assessed, and parasite editing and phenotyping were performed to assess the impact of mutations on parasite resistance. Whole-genome sequencing was performed, and [...] decades ago. Recent studies in East Africa have reported a gradual increase in kelch13 (k13) mutant parasites associated with reduced artesunate efficacy. As part of the Community Access to Rectal Artesunate [...] on. Genome editing confirmed reduced in vitro susceptibility to artemisinin in...
