Strategies for detection of <em>Plasmodium species</em> gametocytes (Publications)
and asexual parasite stages, 18S rRNA transcript-based detection saves efforts and costs. RNA-based positivity is considerably higher than other methods. On the other hand, DNA-based parasite quantification [...] Carriage and density of gametocytes, the transmission stages of malaria parasites, are determined for predicting the infectiousness of humans to mosquitoes. This measure is used for evaluating interventions [...] species prevalence was determined by targeting both, 18S rRNA genes and...
Effectiveness of combined intermittent preventive treatment for children and timely home treatment for malaria control (Publications)
four months (three times in 12 months). Malaria parasite prevalence surveys were conducted before the first and after the third dose of IPTC. RESULTS: Parasite prevalence was reduced from 25% to 3% (p < 0 [...] timely treatment of febrile malaria illness, impacts significantly on the parasite prevalence. The marked reduction in the parasite prevalence with this strategy points to the potential for reducing mala [...] children (IPTC) and timely treatment of malaria-related febrile illness in the...
Re-annotation of the <em>Theileria parva</em> genome refines 53% of the proteome and uncovers essential components of N-glycosylation, a conserved... (Publications)
Comprehensive, accurate parasite genome annotation can facilitate the discovery of novel chemotherapeutic targets for disease treatment, as well as elucidate the biology of the parasite. However, genome annotation [...] sequences in this parasite, and uncovered a minimal N-glycosylation pathway that changes our current understanding of the evolution of this post-translational modification in apicomplexan parasites. [...] BACKGROUND: The apicomplexan parasite Theileria parva causes a livestock...
On-target, dual aminopeptidase inhibition provides cross-species antimalarial activity (Publications)
digestion in asexual parasites. MMV1557817 can kill sexual-stage P. falciparum, is active against murine malaria, and does not show any shift in activity against a panel of parasites resistant to other [...] resistant P. falciparum exhibited a slow growth rate that was quickly outcompeted by wild-type parasites and were sensitized to the current clinical drug, artemisinin. Overall, these results confirm MMV1557817 [...] Their widespread use has led to the development of artemisinin resistance...
TIDE analysis of <em>Cryptosporidium </em>infections by <em>gp60 </em>typing reveals obscured mixed infections (Publications)
is a parasitic disease associated with potentially fatal diarrhea. The most used method in Cryptosporidium subtyping is based on the glycoprotein gene gp60. Each infection can represent a parasite population [...] s. A combination of single oocyst analysis and cloning successfully confirmed the within-sample parasite population diversity. Retrospective sample analysis was conducted on archived chromatograms. RESULTS: [...] epidemiological investigations, also applicable in retrospect. Our...
TIDE analysis of <em>Cryptosporidium </em>infections by <em>gp60 </em>typing reveals obscured mixed infections (Publications)
is a parasitic disease associated with potentially fatal diarrhea. The most used method in Cryptosporidium subtyping is based on the glycoprotein gene gp60. Each infection can represent a parasite population [...] s. A combination of single oocyst analysis and cloning successfully confirmed the within-sample parasite population diversity. Retrospective sample analysis was conducted on archived chromatograms. RESULTS: [...] epidemiological investigations, also applicable in retrospect. Our...
Malaria Gene Regulation (Page)
https://www.swisstph.ch/en/about/mpi/malaria-gene-regulation
important survival strategies of malaria blood-stage parasites - antigenic variation and gametocyte formation. Antigenic variation allows the parasites to evade the human immune response and establish a [...] variation and sexual commitment are absolutely essential for parasite survival. While antigenic variation secures the long-term survival of the parasite population in the bloodstream, sexual commitment provides [...] Early Sexual Differentiation in the Malaria Parasite The SNSF-funded...
Quinuclidine derivatives as potential antiparasitics (Publications)
compounds were also selective for the parasite enzyme rather than the homologous human enzyme. The compounds inhibited the growth of and sterol biosynthesis in Leishmania parasites. In addition, we identified other [...] There is an urgent need for the development of new drugs for the treatment of tropical parasitic diseases such as Chagas' disease and leishmaniasis. One potential drug target in the organisms that cause
Quinuclidine derivatives as potential antiparasitics (Publications)
compounds were also selective for the parasite enzyme rather than the homologous human enzyme. The compounds inhibited the growth of and sterol biosynthesis in Leishmania parasites. In addition, we identified other [...] There is an urgent need for the development of new drugs for the treatment of tropical parasitic diseases such as Chagas' disease and leishmaniasis. One potential drug target in the organisms that cause
Molecular monitoring of the diversity of human pathogenic malaria species in blood donations on Bioko Island, Equatorial Guinea (Publications)
Densities of P. falciparum positive blood donations were ranging from 0.06 to 3707.0 parasites/microL with 79.6% below 100 parasites/microL and therefore not detectable by non-molecular malaria diagnostic tests [...] sub-Saharan Africa, it had been estimated that almost a quarter of blood donations contain malaria parasites. Since rapid diagnostic tests and thick blood smear microscopy lack sensitivity for low density [...] chain reaction. The study was undertaken to determine the prevalence of...
