Questionnaire-based approach to assess schoolchildren's physical fitness and its potential role in exploring the putative impact of helminth and... (Publications)
children's parasitic infection status and self-reported physical fitness was identified. An attrition analysis revealed considerably lower self-reported physical fitness scores of parasitized children who [...] 4%, 74.9%, 54.5%, 14.4% and 1.2%, respectively. High infection intensities and multiple species parasite infections were common. In the 137 children with complete data also from the shuttle run test, we [...] who were excluded from shuttle run testing due to medical concerns in...
Questionnaire-based approach to assess schoolchildren's physical fitness and its potential role in exploring the putative impact of helminth and... (Publications)
children's parasitic infection status and self-reported physical fitness was identified. An attrition analysis revealed considerably lower self-reported physical fitness scores of parasitized children who [...] 4%, 74.9%, 54.5%, 14.4% and 1.2%, respectively. High infection intensities and multiple species parasite infections were common. In the 137 children with complete data also from the shuttle run test, we [...] who were excluded from shuttle run testing due to medical concerns in...
Molecular monitoring of the diversity of human pathogenic malaria species in blood donations on Bioko Island, Equatorial Guinea (Publications)
Densities of P. falciparum positive blood donations were ranging from 0.06 to 3707.0 parasites/microL with 79.6% below 100 parasites/microL and therefore not detectable by non-molecular malaria diagnostic tests [...] sub-Saharan Africa, it had been estimated that almost a quarter of blood donations contain malaria parasites. Since rapid diagnostic tests and thick blood smear microscopy lack sensitivity for low density [...] chain reaction. The study was undertaken to determine the prevalence of...
Diagnosis and Treatment (Page)
https://www.swisstph.ch/en/topics/malaria/diagnosis-and-treatment
different methods of detecting malaria parasites; one can either detect them visually under a microscope, with a rapid test that detects an antigen of the parasite, or via molecular diagnostics. Broad Range [...] Non-Malarial Fevers and Low Parasite Levels in the Amazon One of the outstanding problems in malaria elimination is to provide precise, robust and reliable tools for parasite diagnostics in rural and resource [...] frame of epidemiological studies. Many of the assays to detect and...
The essential malaria protein <em>Pf</em>CyRPA targets glycans to invade erythrocytes (Publications)
studies. Transgenic parasite lines expressing endogenous PfCyRPA with single amino acid exchange mutants indicated that the lectin activity of PfCyRPA has an important role in parasite invasion. Blocking [...] Summary Plasmodium falciparum is a human-adapted apicomplexan parasite that causes the most dangerous form of malaria. P. falciparum cysteine-rich protective antigen (PfCyRPA) is an invasion complex protein [...] with small molecules or with lectin-site-specific monoclonal antibodies can...
Life cycle maintenance and drug-sensitivity assays for early drug discovery in <em>Schistosoma mansoni</em> (Publications)
and promote antischistosomal drug discovery, setting up a drug-screening flow with schistosome parasites remains challenging. Whereas numerous different protocols to obtain and cultivate schistosomes have [...] been published, those describing the drug-screening process are scarce, and none gather together parasite cultivation and early drug discovery procedures. To help overcome this hurdle, we provide here a [...] mansoni life cycle in a laboratory setting, as well as the means of retrieving...
Analysis of subtelomeric virulence gene families in <em>Plasmodium falciparum</em> by comparative transcriptional profiling (Publications)
Plasmodium falciparum genome is equipped with several subtelomeric gene families that are implicated in parasite virulence and immune evasion. Members of these families are uniformly positioned within heterochromatic [...] to variegated expression. The best-studied example is that of the var family encoding the major parasite virulence factor P. falciparum erythrocyte membrane protein 1 (PfEMP1). PfEMP1 undergoes antigenic [...] role in endogenous gene transcription by comparative genome-wide...
Critical roles of glycosylphosphatidylinositol for <em>Trypanosoma brucei</em> (Publications)
TbGPI10; therefore, GPI synthesis is essential for growth of mammalian stage parasites. Procyclic form cells (insect stage parasites) lacking the surface coat proteins because of disruption of TbGPI10 are viable [...] Trypanosoma brucei, the protozoan parasite responsible for sleeping sickness, evades the immune response of mammalian hosts and digestion in the gut of the insect vector by means of its coat proteins tethered [...] cell surface via glycosylphosphatidylinositol (GPI) anchors. To...
7-<em>N</em>-substituted-3-oxadiazole quinolones with potent antimalarial activity target the cytochrome <em>bc</em>1 complex (Publications)
antimalarial chemotypes, we screened the Janssen Jumpstarter library against the P. falciparum asexual parasite and identified the 7-N-substituted-3-oxadiazole quinolone hit class. We established the structu [...] pyrimidine biosynthesis and an established antimalarial target. Profiling against drug-resistant parasites confirmed that WJM228 confers resistance to the Q(o) site but not Q(i) site mutations, and in a [...] of cyt b. Consistent with other cyt b targeted antimalarials, WJM228...
Bottlenecks and the maintenance of minor genotypes during the life cycle of <em>Trypanosoma brucei</em> (Publications)
minimum of several hundred parasites survived transmission from a tsetse fly to a mouse, or vice versa, and contributed to the infection in the new host. In contrast, the parasites experienced a pronounced [...] African trypanosomes are digenetic parasites that undergo part of their developmental cycle in mammals and part in tsetse flies. We established a novel technique to monitor the population dynamics of [...] sequences in a non-transcribed region of the genome. Infections were initiated...
