Challenges in malaria management and a glimpse at some nanotechnological approaches (Publications)
provides tools to design strategies to target drug molecules to specific stages of the parasite, treat drug-resistant parasites, resolve severe malaria, increase vaccine efficacies and combinations thereof. This [...] past years, there are still considerable challenges to be solved. To name a few, drug-resistant parasites, insecticide-resistant mosquitoes and the difficulty to formulate a potent malaria vaccine need
Novel 1-methyl-1 <em>H</em>-pyrazole-5-carboxamide derivatives with potent anthelmintic activity (Publications)
selectivity toward the parasite when tested in vitro against the human MCF10A cell line. In addition, compounds 9 and 27 showed promising activity against a panel of other parasitic nematodes, including [...] screen of two different libraries of small molecules against the motility and development of the parasitic nematode Haemonchus contortus led to the identification of two 1-methyl-1 H-pyrazole-5-carboxamide
Characterization of a serine hydrolase targeted by acyl-protein thioesterase inhibitors in <em>Toxoplasma gondii</em> (Publications)
Treatment of parasites with low micromolar concentrations of beta-lactone- or triazole urea-based inhibitors that target human APT1 showed varied detrimental effects at multiple steps of the parasite lytic cycle [...] contributes to key biological processes in Apicomplexa with the recent palmitome of the malaria parasite Plasmodium falciparum reporting over 400 substrates that are modified with palmitate by a broad [...] similar to APTs, and homology modeling points toward an APT-like enzyme....
<em>In vitro</em> and<em> in vivo</em> activity of Solithromycin (CEM-101) against <em>Plasmodium</em> species (Publications)
With the emergence of Plasmodium falciparum infections exhibiting increased parasite clearance times in response to treatment with artemisinin-based combination therapies, the need for new therapeutic [...] in development, has been shown to be an effective, broad-spectrum antimicrobial agent. Malarial parasites possess an unusual organelle, termed the apicoplast, which carries a cryptic genome of prokaryotic [...] macrolide azithromycin, demonstrate a "delayed death" effect, whereby treated...
Induction of humoral immune response to multiple recombinant <em>Rhipicephalus appendiculatus</em> antigens and their effect on tick feeding success... (Publications)
natural host-tick-parasite combination. In spite of evidence of strong immune responses to all of the antigens in the cocktail, vaccination with this combination of tick and parasite antigens did not appear [...] appendiculatus feeding success and possibly reduce tick-transmission of T. parva in a natural host-tick-parasite challenge model. METHODS: Cattle were inoculated with a multivalent antigen cocktail containing [...] anti-tick vaccine candidates in biologically relevant challenge systems...
Emergence of artemisinin-resistant <em>Plasmodium falciparum</em> with <em>kelch13 </em>C580Y mutations on the island of New Guinea (Publications)
kelch13 C580Y, which have affected the parasites' drug sensitivity. This worrying development reinforces the need for increased surveillance of the evolving parasite populations on the island, to contain [...] genotyped clinical samples. One of these mutants exhibited the highest survival rate (6.8%) among all parasites surveyed in ring-stage survival assays (RSA) for artemisinin. Analyses of kelch13 flanking regions [...] descent (IBD) showed that multiple portions of the mutants' genomes...
Blood-stage parasitaemia and age determine <em>Plasmodium falciparum</em> and <em>P. vivax</em> gametocytaemia in Papua New Guinea (Publications)
gametocytes by detection of pfs25 and pvs25 mRNA transcripts by reverse-transcriptase PCR (qRT-PCR). The parasite prevalence by PCR was 18.5% for Plasmodium falciparum and 13.0% for P. vivax. 52.5% of all infections [...] in 60% of P. falciparum-positive and 51% of P. vivax-positive samples. Each 10-fold increase in parasite density led to a 1.8-fold and 3.3-fold increase in the odds of carrying P. falciparum and P. vivax [...] gametocyte positive and gametocyte densities was highest in young...
Resisting infection by <em>Plasmodium berghei</em> increases the sensitivity of the malaria vector <em>Anopheles gambiae</em> to DDT (Publications)
sporozoites (the infectious stage of the Plasmodium parasite) and they are relatively old, as they need to survive the development period of the malaria parasite. This study examines the effects of infection [...] mosquitoes that had fed on Plasmodium-infected mice than in those that had not been exposed to the parasite. The latter effect was mainly due to the high sensitivity of mosquitoes that had fed on an infected [...] those that had fed on an uninfected mouse. CONCLUSIONS: The observed...
Discovery of dual function acridones as a new antimalarial chemotype (Publications)
an immutable and vulnerable target, because it is not parasite-encoded and its detoxification during haemoglobin degradation, critical to parasite survival, can be subverted by drug-haem interaction as [...] 'verapamil-like' chemosensitization to chloroquine and amodiaquine against quinoline-resistant parasites, T3.5 also results in an apparently mechanistically distinct synergism with quinine and with piperaquine [...] piperaquine. This synergy, evident in both quinoline-sensitive and...
Emergence of artemisinin-resistant <em>Plasmodium falciparum</em> with <em>kelch13 </em>C580Y mutations on the island of New Guinea (Publications)
kelch13 C580Y, which have affected the parasites' drug sensitivity. This worrying development reinforces the need for increased surveillance of the evolving parasite populations on the island, to contain [...] genotyped clinical samples. One of these mutants exhibited the highest survival rate (6.8%) among all parasites surveyed in ring-stage survival assays (RSA) for artemisinin. Analyses of kelch13 flanking regions [...] descent (IBD) showed that multiple portions of the mutants' genomes...
