MAHRP2, an exported protein of <em>Plasmodium falciparum</em>, is an essential component of Maurer's cleft tethers (Publications)
Summary Upon invasion into erythrocytes, the malaria parasite Plasmodium falciparum must refurbish the host cell. The objective of this study was to elucidate the location and function of MAHRP2 in these [...] structures tether organelles known as Maurer's clefts to the erythrocyte skeleton. Live cell imaging of parasite transfectants expressing MAHRP2-GFP revealed both mobile and fixed populations of the tether-like [...] Several attempts to delete the mahrp2 gene failed indicating that...
An Alba-domain protein required for proteome remodelling during trypanosome differentiation and host transition (Publications)
The transition between hosts is a challenge for digenetic parasites as it is unpredictable. For Trypanosoma brucei subspecies, which are disseminated by tsetse flies, adaptation to the new host requires [...] stumpy forms from translational repression and stimulating polysome formation. In its absence, parasites fail to remodel their proteome appropriately, lack components of the mitochondrial respiratory chain [...] postulate that Alba-domain proteins play similar roles in regulating...
Overcoming roadblocks in the development of vaccines for leishmaniasis (Publications)
s represent a group of parasitic diseases caused by infection with one of several species of Leishmania parasites. Disease presentation varies because of differences in parasite and host genetics and may
Infection of organotypic slice cultures from rat central nervous tissue with <em>Trypanosoma brucei brucei</em> (Publications)
we have used this in vitro approach to study the complex interactions between host tissue and parasites during late stages of cerebral African trypanosomiasis. Light and electron microscopical studies [...] severely affected even after several weeks of trypanosome infection. The presence of a large number of parasites does not seem to be deleterious to neuronal survival. Secondly, most of the trypanosomes are located [...] cytoplasm of glial cells, which in some cases were identified as...
Infection of organotypic slice cultures from rat central nervous tissue with <em>Trypanosoma brucei brucei</em> (Publications)
we have used this in vitro approach to study the complex interactions between host tissue and parasites during late stages of cerebral African trypanosomiasis. Light and electron microscopical studies [...] severely affected even after several weeks of trypanosome infection. The presence of a large number of parasites does not seem to be deleterious to neuronal survival. Secondly, most of the trypanosomes are located [...] cytoplasm of glial cells, which in some cases were identified as...
Overcoming roadblocks in the development of vaccines for leishmaniasis (Publications)
s represent a group of parasitic diseases caused by infection with one of several species of Leishmania parasites. Disease presentation varies because of differences in parasite and host genetics and may
An Alba-domain protein required for proteome remodelling during trypanosome differentiation and host transition (Publications)
The transition between hosts is a challenge for digenetic parasites as it is unpredictable. For Trypanosoma brucei subspecies, which are disseminated by tsetse flies, adaptation to the new host requires [...] stumpy forms from translational repression and stimulating polysome formation. In its absence, parasites fail to remodel their proteome appropriately, lack components of the mitochondrial respiratory chain [...] postulate that Alba-domain proteins play similar roles in regulating...
MAHRP2, an exported protein of <em>Plasmodium falciparum</em>, is an essential component of Maurer's cleft tethers (Publications)
Summary Upon invasion into erythrocytes, the malaria parasite Plasmodium falciparum must refurbish the host cell. The objective of this study was to elucidate the location and function of MAHRP2 in these [...] structures tether organelles known as Maurer's clefts to the erythrocyte skeleton. Live cell imaging of parasite transfectants expressing MAHRP2-GFP revealed both mobile and fixed populations of the tether-like [...] Several attempts to delete the mahrp2 gene failed indicating that...
<em>In vitro</em> assessment of the pharmacodynamic properties and the partitioning of OZ277/RBx-11160 in cultures of <em>Plasmodium falciparum</em> (Publications)
to OZ277 were monitored by incorporation of [(3)H]hypoxanthine into parasite nucleic acids and by light-microscopic analysis of parasite morphology. Partitioning studies were performed with radiolabelled [...] OBJECTIVES: Using synchronous cultures of Plasmodium falciparum malaria, the stage sensitivity of the parasite to OZ277 (RBx-11160), the first fully synthetic antimalarial peroxide that has entered Phase II
Click coupling of flavylium dyes with plasmodione analogues: towards new redox-sensitive pro-fluorophores (Publications)
suggests the efficacy of the fluorescent flavylium dyes in P. falciparum-parasitized red blood cells, paving the way for future parasite imaging studies to monitor cellular redox processes. [...] tive molecular fluorescent probes for the detection of redox changes in Plasmodium falciparum-parasitized red blood cells remains of interest due to the limitations of current genetically encoded biosensors
