We acquire new functional and mechanistic insight into processes linked to the essential biology of malaria blood stage parasites. These parasite forms are responsible for all malaria-related morbidity and mortality as well as for the transmission of parasites between humans via the mosquito vector. We place particular emphasis on
- Investigating the molecular mechanisms underlying parasite-induced red blood cell remodeling, through which infected red blood cells adopt pathogenic traits
- Understanding the transcriptional and epigenetic mechanisms responsible for antigenic variation and sexual conversion, which facilitate chronic blood infection and parasite transmission, respectively; and
- The identification and characterization of parasite ligands required for red blood cell invasion and their cognate invasion-inhibitory antibodies.
We further engage in elucidating mechanisms of action of anti-malarial drugs and in identifying immunological response signatures associated with native and adaptive anti-malarial immunity in vivo.
In this projects we study the interaction network of exported proteins which is of crucial importance for the export of virulence factors to the surface of the infected host cells and for other modifications allowing the parasite’s survival in its host cell. We use state-of-the-art molecular-genetic and cell-biological methods and produce a substantial number of transgenic parasites. In these parasites we study the function of exported proteins in order to identify those interactions that are needed to elicit the most important modifications.
The project aims to understand the processes responsible for the differentiation of asexual malaria blood stage parasites into sexual forms. The anticipated results may open up new avenues for the development of transmission-blocking drugs and vaccines.
A reverse vaccinology approach has led us to the identification of the P. falciparum Cysteine Rich Protective Antigen (PfCyRPA). The protein forms together with PfRH5 and PfRipr a multiprotein complex crucial for erythrocyte invasion. We have determined the crystal structure of PfCyRPA and are using this and parasite inhibitory and non-inhibitory anti-CyRPA monoclonal antibodies as a basis for analyzing its function.
Related PublicationsAll Publications
Gockel J, Voss T.S, Bártfai R. The troubled puberty of malaria parasites. Trends Parasitol. 2023;39(3):155-157. DOI: 10.1016/j.pt.2023.01.006
Kuehnel R.M et al. A Plasmodium membrane receptor platform integrates cues for egress and invasion in blood forms and activation of transmission stages. Sci Adv. 2023;9(24):eadf2161. DOI: 10.1126/sciadv.adf2161
Meier L et al. Repurposing know-how for drug development: case studies from the Swiss Tropical and Public Health Institute. Chimia. 2023;77(9):582-592. DOI: 10.2533/chimia.2023.582
Ruiz J.L et al. From contigs towards chromosomes: automatic improvement of long read assemblies (ILRA). Brief Bioinform. 2023;24(4):1-11. DOI: 10.1093/bib/bbad248
Thommen B.T et al. Genetic validation of PfFKBP35 as an antimalarial drug target. Elife. 2023;12. DOI: 10.7554/eLife.86975