Multiplex real-time PCR for the diagnosis of malaria: correlation with microscopy (Publications)
the agreement between PCR-based identification and microscopy and (ii) the correlation between the parasite load as determined by quantitative PCR and by microscopy. For 83 patients positive by microscopy [...] first EDTA-blood sample was tested by multiplex PCR to confirm smear-based species identification. Parasite load was assessed daily using both microscopy and PCR. Among the 83 patients tested, one was positive [...] three species and were attributed to inaccurate morphological...
8,8-dialkyldihydroberberines with potent antiprotozoal activity (Publications)
nM against these three parasites, respectively. In turn, two 8,8-dialkylcanadines, obtained by reduction of the corresponding 8,8-DDBs, were much less potent against these parasites in vitro. While the natural [...] 8-DDBs) were found to possess mid- to low-nanomolar potency against Plasmodium falciparum blood-stage parasites, Leishmania donovani intracellular amastigotes, and Trypanosoma brucei brucei bloodstream forms
Multiplex real-time PCR for the diagnosis of malaria: correlation with microscopy (Publications)
the agreement between PCR-based identification and microscopy and (ii) the correlation between the parasite load as determined by quantitative PCR and by microscopy. For 83 patients positive by microscopy [...] first EDTA-blood sample was tested by multiplex PCR to confirm smear-based species identification. Parasite load was assessed daily using both microscopy and PCR. Among the 83 patients tested, one was positive [...] three species and were attributed to inaccurate morphological...
Proteomic analysis of exported chaperone/co-chaperone complexes of <em>P. falciparum</em> reveals an array of complex protein-protein interactions (Publications)
Malaria parasites modify their human host cell, the mature erythrocyte. This modification is mediated by a large number of parasite proteins that are exported to the host cell, and is also the underlying [...] weight protein complexes. These complexes are found both in the infected erythrocyte, and within the parasite-derived compartment referred to as the parasitophorous vacuole. Surprisingly, our data also reveal
Helminth Immunology | Swiss TPH (Page)
https://www.swisstph.ch/en/about/mpi/helminth-immunology
molecular events involved in parasite evasion of host immune attack, as well as weaknesses in parasite development that can be targeted with drugs. We study helminth parasites, because they are fascinating [...] three axis of research: Deciphering host parasite/interactions: from microbiome to pathology Developing novel anthelmintics and vaccine Understanding parasites development through molecular approaches [...] helminths have been hindered by the difficulty of accessing large quantity of par...
Design and pre-clinical profiling of a <em>Plasmodium falciparum</em> MSP-3 derived component for a multi-valent virosomal malaria vaccine (Publications)
falciparum cross-reactive antibodies. RESULTS: While all MSP-3-derived peptides were able to elicit parasite-binding antibodies, stabilization of turn structures by cyclization had no immune-enhancing effect [...] rabbits tested seroconverted and consistently elicited antibodies that interacted with blood stage parasites. It was observed that a dose of 50 microg was superior to a dose of 10 microg and that influenza [...] Good Manufacturing Practice (GMP), was tested in mice and rabbits. All...
Prevalence of <em>Fasciola gigantica</em> infection in slaughtered animals in south-eastern Lake Chad area in relation to husbandry practices and... (Publications)
classified as lightly infected with 1-10 parasites, 18% (n =36) as moderately infected with 11-100 parasites and 29% (n = 58) as heavily infected with more than 100 parasites per animal.Animals grazing close to [...] accounts, but the latest reports from Chad are from the 1970s. Mobile pastoralists perceive liver parasites as a significant problem and think that proximity to Lake Chad can lead to infection. This study
Design and pre-clinical profiling of a <em>Plasmodium falciparum</em> MSP-3 derived component for a multi-valent virosomal malaria vaccine (Publications)
falciparum cross-reactive antibodies. RESULTS: While all MSP-3-derived peptides were able to elicit parasite-binding antibodies, stabilization of turn structures by cyclization had no immune-enhancing effect [...] rabbits tested seroconverted and consistently elicited antibodies that interacted with blood stage parasites. It was observed that a dose of 50 microg was superior to a dose of 10 microg and that influenza [...] Good Manufacturing Practice (GMP), was tested in mice and rabbits. All...
Prevalence of <em>Fasciola gigantica</em> infection in slaughtered animals in south-eastern Lake Chad area in relation to husbandry practices and... (Publications)
classified as lightly infected with 1-10 parasites, 18% (n =36) as moderately infected with 11-100 parasites and 29% (n = 58) as heavily infected with more than 100 parasites per animal.Animals grazing close to [...] accounts, but the latest reports from Chad are from the 1970s. Mobile pastoralists perceive liver parasites as a significant problem and think that proximity to Lake Chad can lead to infection. This study
Basic Research in Infection Biology | Swiss TPH (Page)
https://www.swisstph.ch/en/research/basic-research-in-infection-biology
malaria cases. P. falciparum blood-stage parasites are our main subjects of study. We are investigating several key aspects of the biology of these intracellular parasites, such as erythrocyte invasion and [...] in Infection Biology Infectious diseases are caused by pathogens - viruses, bacteria, fungi or parasites - that invade and multiply in our bodies or colonise our skin or mucosal surfaces. Pathogens infect [...] bioinformatics. Swiss TPH has broad and interdisciplinary scientific...
